存活蛋白在子宫内膜癌中的表达及其在细胞凋亡中的作用  

Expression of survivin in endometrial cancer and its role in regulating apoptosis of endometrial cancer cells

作　　者：艾志宏[1] 丰有吉[2] 

机构地区：[1]上海交通大学附属第六人民医院妇产科,上海200233 [2]复旦大学附属妇产科医院妇科,上海200011

出　　处：《肿瘤》2008年第8期685-688,共4页Tumor

摘　　要：目的:探讨子宫内膜癌中存活蛋白(survivin)的表达及其对细胞凋亡的作用。方法:免疫组织化学法检测survivin在子宫内膜癌组织中的表达,并且应用RNA干扰技术沉默survivin基因,RT-PCR和Western印迹法检测RNA干扰效果,FCM法检测RNA干扰前后细胞凋亡的变化,Western印迹法检测凋亡相关蛋白caspase-3、caspase-8和bcl-2的表达变化。结果:子宫内膜癌组织中survivin蛋白的阳性率显著高于不典型增生和正常内膜组织(P<0.05和P<0.01)。RNA干扰有效抑制了survivin mRNA和蛋白的表达(均为P<0.01),并且诱导了细胞凋亡,2个干扰组的细胞凋亡率均显著高于对照组(P<0.01)。RNA干扰亦上调了活性caspase-3、caspase-8的表达。结论:survivin基因的异常表达与子宫内膜癌的发生密切相关,抑制其表达可以诱导子宫内膜癌细胞发生凋亡。Objective ：To explore the expression of survivin in endometrial cancer and its roles in regulating apoptosis of endometrial cancer cells. Methods： Immunohistochemistry was used to examine the expression of survivin in endometrial cancer specimens. RNA interference （RNAi） was applied to knock down survivin expression. The effect of RNAi was examined by RT-PCR and Western blotting. Cell apoptosis was detected by the FCM before and after RNAi. Western blotting was used to detect the expressions of caspase-3, caspase-8 and Bcl-2, which were closely associated with apoptosis. Results：The positive rate of survivin protein in endometrial cancer was obviously higher than those in atypical endometrium and normal endometrium （ P 〈 0.05 and P 〈 0.01, respectively）. RNAi effectively inhibited the expressions of survivin mRNA and survivin protein （P 〈 0.01 ）. RNAi also induced cell apoptosis. Cell apoptosis rates in the two groups of RNAi were obviously higher than those in control groups（ P 〈 0.01 ）. Survivin RNAi also up-regulated the cleavage of caspaseo3 and caspase-8. Conclusion：The aberrant expression of survivin may be closely related to the carcinogenesis of endometrial cancer, and inhibition of its expression may induce apoptosis of endometrial cancer cells.

关 键 词：子宫内膜肿瘤 凋亡抑制蛋白质类 基因沉默 细胞凋亡 存活蛋自 

分 类 号：R711.74[医药卫生—妇产科学]