蛋白质和磷酸水解酶在废水处理系统中的活性和作用  

Activities and Actions of Protein and Polyphosphate Hydrolytic Enzymes in Wastewater Treatment

作　　者：李茵[1] 罗翠[1] 沈国[1] 

机构地区：[1]东华大学环境科学与工程学院,上海201620

出　　处：《应用与环境生物学报》2008年第4期514-517,共4页Chinese Journal of Applied and Environmental Biology

基　　金：上海市自然科学基金(06ZR14002);上海市重点学科建设项目(B604)~~

摘　　要：通过分析亮氨酸氨基肽酶和碱性磷酸酶,研究了厌氧–缺氧–好氧废水处理系统中蛋白质和磷酸水解酶的活性及其作用.结果表明,亮氨酸氨基肽酶在缺氧和好氧池中的活性分别达19.2μmolL-1h-1和21.6μmolL-1h-1;而碱性磷酸酶在厌氧池中占主导地位,平均活性达32.5μmolL-1h-1,好氧池中的活性只有厌氧池的一半,缺氧池中的酶活性最小.动力学研究得到,厌氧条件碱性磷酸酶的最大反应速度vmax为778.42μmolL-1h-1,是亮氨酸氨基肽酶vmax值的一百倍以上;亮氨酸氨基肽酶的vmax值在厌氧、缺氧、好氧池中依次增大.此外,外加的NO2-、NO3-离子对亮氨酸氨基肽酶和PO43-离子对碱性磷酸酶的活性均有不同程度的促进作用,只有浓度为50mg/L的PO43-对碱性磷酸酶有显著的抑制.Activities and actions of protein and polyphosphate hydrolytic enzymes in an anaerobic-anoxic-aerobic wastewater treatment system were investigated by analyses of leucine-aminopeptidase （L-AMP） and alkaline phosphatase （APA）. The results showed that the L-AMP activities in the anoxic and aerobic reactors reached 19.2 μmolL^-1h^-1 and 21.6 μmolL^-1h^-1, respectively. The APA displayed predominant activity （32.5 μmolL^-1h^-1） in the anaerobic reactor. The activity in the aerobic reactor only accounted for half of that in the anaerobic reactor and that in anoxic reactor was the lowest. Kinetic study showed that the maximum reaction velocity （vmax） of APA in the anaerobic reactor was 778.42 μmolL^-1h^-1, which was over one hundred times higher than that of L-AMP. The vmax of L-AMP increased in order of the anaerobic, anoxic and aerobic reactors. In addition, the added NO2^-, NO3^- and PO4^3- ions could stimulate the activities of L-AMP and APA to different extent, except that only 50 mg/L PO4^3- had obvious inhibition effect on APA activity. Fig 4, Tab 2, Ref 17

关 键 词：亮氨酸氨基肽酶 碱性磷酸酶 酶活性 厌氧-缺氧-好氧 废水处理 

分 类 号：X703[环境科学与工程—环境工程]