紫外线致弱尾蚴免疫猪血清筛选日本血吸虫成虫cDNA文库  

Immunoscreening of Schistosoma japonicum Adult Worm cDNA Library by Serum from Swine Vaccinated with Ultraviolet Attenuated Cercariae

作　　者：柳建发[1] 汤治元[1] 刘玉新[1] 杨淑娟[1] 孟睿[2] 吴海玮[2] 

机构地区：[1]宁波大学医学院,浙江宁波315211 [2]南京医科大学病原生物系,南京210029

出　　处：《地方病通报》2008年第4期18-19,22,共3页Endemic Diseases Bulletin

基　　金：宁波市自然科学基金资助(2007313)

摘　　要：目的寻找紫外线致弱日本血吸虫尾蚴中起免疫保护作用的候选抗原分子,为血吸虫疫苗研究提供新的候选靶位抗原。方法用紫外线照射致弱尾蚴免疫猪血清筛选日本血吸虫成虫cDNA文库,测定用于筛选的cDNA文库滴度,选用最佳滴度的cDNA文库用于筛库。结果测得最佳cDNA文库的滴度为1.89×109ρfu/mL,经3轮筛选,致弱尾蚴组分别获得20个、10个阳性克隆,经3轮筛选获7个阳性克隆;对照尾蚴组获15个和9个阳性克隆,经3轮筛选获3个阳性克隆;无尾蚴感染组未获阳性克隆。结论获得的阳性克隆可能为寻找编码抗日本血吸虫感染的抗原基因提供了材料与方向。Objective To explore the antigenic molecules of ultraviolet radiated attenuated cercariae of Schistosoma japonicum,and get some future candidate antigens for developing vaccine and controlling measure against schistosomiasis.Methods Adult worm cDNA library of Schistosoma japonicum was screened by serum of swine vaccinated with ultraviolet attenuated cercariae,and the best cDNA library which was used for screening was detected titer.Results The best titer of cDNA library for screening was 1.89×10^9ρfu/mL,according to three rounds of screening,20,10 and 7 positive clones were obtained respectively at attenuated cercariae group,and at control cercariae group,15,9 and 3 positive clones were screened,but at normal serum group,no positive clones were screened.Conclusions The positive clones obtained maybe were the genes encoding the antigens which could provide protective immunity against schistosomiasis and elicit the directions for antigenic genes encoding antigen against Schistosoma japonicum.

关 键 词：紫外线 致弱尾蚴 免疫猪血清 筛选 日本血吸虫 CDNA文库 

分 类 号：R383.24[医药卫生—医学寄生虫学] Q342.2[医药卫生—基础医学]