乌司他丁(天普洛安)对大鼠放射性肺损伤过程中TGF-β1的影响  

Effect of ulinastatin on transforming growth factor β1 in rats with radiation-induced lung injury

作　　者：保鹏涛[1] 戚好文[1] 杨善潮[2] 李艳[1] 张力江[1] 吴昌归[1] 高维[3] 

机构地区：[1]第四军医大学西京医院呼吸内科,陕西西安710033 [2]第四军医大学西北核技术研究所,陕西西安710024 [3]第四军医大学唐都医院呼吸内科,陕西西安710038

出　　处：《第四军医大学学报》2008年第16期1462-1464,共3页Journal of the Fourth Military Medical University

摘　　要：目的:探讨乌司他丁(天普洛安)对大鼠放射性肺损伤过程中TGF-β1水平的影响,探讨其在治疗放射性肺损伤中的作用.方法:60只雌性SD大鼠随机分为3组:对照组(C组)、单纯照射组(R组)、乌司他丁治疗组(U组).R及U组大鼠行直线加速器全胸部照射一次,剂量为20Gy.照射后U组通过尾静脉注射乌司他丁[10000U/(kg·d)],共计7d;C,R组注射同等体积的生理盐水.于照射后7,15,30,60d处死动物,取部分组织行HE染色及Masson染色观察组织学及肺胶原蛋白等,另取部分组织行免疫组化染色观察TGF-β1阳性细胞;使用酶联免疫吸附分析法检测肺泡灌洗液和血清中TGF-β1水平.结果:R,U组在照射后肺间质密度、TGF-β1阳性面积以及肺泡灌洗液和血清中TGF-β1水平随时间增加而逐渐升高,其中以R组30,60d时与C组比较最为明显(P<0.01),同时可以发现U组与同期R组相比较具有统计学意义(P<0.01);肺泡灌洗液和血清中TGF-β1水平变化与肺组织病理学变化趋势基本一致.结论:TGF-β1在放射性肺损伤发病机制中起到重要作用,间接地反应了放射性肺损伤程度,乌司他丁能通过显著降低TGF-β1的水平,从而抑制炎症反应的发生与发展,有效地防护放射性肺损伤.AIM： To observe the effect of ulinastatin on the expression level of transforming growth factor β1 （ TGF-β1 ） in radiation-induced lung injury and to investigate its roles in treatment of radiation-induced lung injury. METHODS： Sixty female SD rats were divided into 3 groups： normal control group （group C）, radiation group （ group R ）, Ulinastatin treatment group （ group U）. Rats in group R and U were irradiated with linear accelerator at a single dose of 20 Gy. Rats in group U received injection of ulinastatin at a dose of 10 000 u/kg · d for 7 days through vena caudalis immediately after the irradiation and rats in group C and R received the same volume of normal saline. Rats were killed on 7, 15, 30, 60 days. Sections of lung tissues were observed by HE staining and Masson staining. Expressions of TGF-β1 in lung, BALF and serum were determined by immunohistochemistry and ELISA. RESULTS： Densities of lung mesenchyma, positive expression area and concentrations of TGF-β1 gradually increased after irradiation, especially on day 30, 60 in group R. Difference between group R and C was statistically significant （P 〈 0.01 ）. Additionally, difference between group U and R was also statistically significant （ P 〈 0.01 ）. Variations of TGF-β1 in BALF and serum were accordant with the pathological changes in lung after irradiation. CONCLUSION： TGF-β1 plays an important role in radiation-induced lung injury and indirectly reflects the extent of radiation-induced lung injury. Ulinas-tatin could prevent the inflammatory process and radiation-induced lung injury effectively by inhibiting the level of TGF-β1.

关 键 词：乌司他丁 转化生长因子Β1 肺 辐射损伤 

分 类 号：R562.25[医药卫生—呼吸系统]