一阶导数分光光度法测定复方蛋黄乳膏中VitB_2的含量  

Quantitative determination of VitB_2 in the compound yolk emulsion by the first order derivative spectrophotometry

作　　者：罗兰[1] 林筱琦[1] 阿不都克依木.扎依提 胡梦颖[2] 

机构地区：[1]新疆医科大学第一附属医院药剂科,新疆乌鲁木齐830011 [2]新疆医科大学药学院,2001级新疆乌鲁木齐830011

出　　处：《新疆医科大学学报》2008年第6期660-661,共2页Journal of Xinjiang Medical University

摘　　要：目的：建立复方蛋黄乳膏中VitB2的含量测定方法。方法：采用一阶导数分光光度法测定复方蛋黄乳膏中的VitB2含量,VitB2的检测波长范围为386-554 nm。结果：浓度范围为0.011 9-0.023 8 mg/ml,测定振幅D与浓度呈良好的线性关系（γ=0.999 2）,回收率为99.4%,RSD为0.4%。结论：本法可消除其它组分的干扰,简便、易行、准确,适合医院制剂的质量控制。Objective： To establish a method to determine content of VitB2 in the compound yolk emulsion by the first order derivative spectrophotometry. Methods： The first order derivative spectrophotometry was used to determine content of VitB2 in the compound yolk emulsion. Results： The linear correlation coeffi- cient of the amplitude （D） at 386-554 nm with concentration of VitB2 in the compound yolk emulsion was 0. 999 2 in the range of 0. 011 9-0. 023 8 mg/ml,the average recovery of VitB2 was 99.41% （n =6）, the relative standard deviation （RSD） of which was 0. 42%Conclusion： The interferences of the other components can be excluded by this method; which is simple, rapid and accurate. It is suitable for the quality control of hospital preparation.

关 键 词：一阶导数分光光度法 复方蛋黄乳膏 VITB2 

分 类 号：R988.2[医药卫生—药品] R297.2[医药卫生—药学]