对沙眼衣原体感染细胞促凋亡蛋白表达的影响  

Effects of MG132 on Proapoptotic Proteins in Chlamydia trachomatis-infected Cell

作　　者：杨志英[1] 陈福春[1] 欧录明[1] 余晓芬[1] 曹艳华[1] 阳帆[1] 

机构地区：[1]湘南学院基础医学课部,湖南郴州423000

出　　处：《中国医药导报》2008年第24期11-12,共2页China Medical Herald

基　　金：湖南省教育厅基金项目(批准号06C787)

摘　　要：目的:研究蛋白酶体抑制剂MG132对沙眼衣原体感染细胞促凋亡蛋白Bim、Puma表达的影响,探讨沙眼衣原体抑制宿主细胞凋亡的可能机制。方法:以沙眼衣原体(D血清型)感染Hela229细胞,用不同浓度MG132作用后,Western-blot检测Bim、Puma蛋白质表达水平的变化。结果:沙眼衣原体感染12h后Bim、Puma的表达降低;24h后完全消失。MG132能抑制Bim、Puma的表达下降。结论:沙眼衣原体感染Hela229细胞后可降低促凋亡蛋白Bim、Puma蛋白质的表达;MG132可阻止这一作用,Bim和Puma的降解依赖蛋白酶体的活性。Objective：To explore the effects of MG132 on Proapoptotic Proteins Bim,Puma and the mechanism of apoptotic inhibition in Chlamydia trachomatis-infected Hela229 cells. Methods：The experiments cell were treated with the proteasome inhibitor MG132. Western-blot was applied to detect the expression of Bim,Puma at protein level. Results：Bim, Puma were degraded approximately 12 h after infection with C. trachomatis and disappeared at 24 h. MG132 can inhibit the degradation. Conclusion：The levels of Bim,Puma were reduced by infection with C. trachomotis; the reduce was prevented by MG132. Degradation of Bim,Puma requires proteasomal activity.

关 键 词：沙眼衣原体(D血清型) HELA229细胞 Bim PUMA 细胞凋亡 

分 类 号：R691.3[医药卫生—泌尿科学] R730.23[医药卫生—外科学]