蒺藜苜蓿子叶节高频再生系统的建立  

Establishment of high frequency regeneration system of Medicago truncatula with cotyledonary-node explants

作　　者：冯利兴[1] 傅力[1] 刘伟[2] 孙杰[2] 陈爱民[3] 王彦章[3] 

机构地区：[1]新疆农业大学食品科学学院,新疆乌鲁木齐830000 [2]石河子大学农学院新疆生产建设兵团绿洲生态农业重点实验室,新疆石河子832000 [3]中国科学院上海生命科学研究院植物生理生态研究所植物分子遗传国家重点实验室,上海200032

出　　处：《草业科学》2008年第8期52-56,共5页Pratacultural Science

基　　金：新疆生产建设兵团绿洲生态农业重点实验室开放课题(200503);中国科学院上海生命科学研究院植物生理生态研究所探索性课题

摘　　要：通过直接的芽器官发生途径,建立了蒺藜苜蓿Medicago truncatula子叶节外植体的高频植株再生体系。选用在高达5 mg/L 6-苄氨基嘌呤(6-BA)的种子萌发培养基上生长3 d的蒺藜苜蓿幼苗,制备获得由1片子叶和半个胚轴组成的子叶节外植体。以SH培养基为基本培养基,对不同浓度α-萘乙酸(NAA)和6-BA组合诱导不定芽形成条件进行优化的结果表明,不定芽诱导培养基中仅用浓度为4 mg/L 6-BA的平均不定芽数达到3.56,显著优于与其它生长调节物质组合的处理。The high frequency plant regeneration system of Medicago truncatula with cotyledonary-node explants was developed through direct shoot organogenesis. The three day old seedlings grown in medium supplemented with 5 mg/L 6-BA were used to prepare the cotyledonary-node explants. The cotyledonary-node explants had one cotyledon and half of the embryonic axis. Based on SH basal medium, the combination of 6-BA and NAA concentrations was optimized for shoot induction. The results indicated that the combination added 4 mg/L 6-BA in shoot induction medium could obtain 3.56 shoots, which was the best.

关 键 词：蒺藜苜蓿 子叶节外植体 植株再生 

分 类 号：S541.9[农业科学—作物学]