单纯血管束、神经束分别植入组织工程骨修复兔骨缺损对降钙素基因相关肽和神经肽Y表达的影响  

A study of the different effect on the expression of calcitonin gene related peptide and neuropeptide Y in tissue engineered bone with vascular bundle graft in vivo and that with sensory nerve tract graft in vivo

作　　者：崔建德[1] 裴国献[1] 江汕[1] 戴金良[1] 刘勇[1] 姚旺祥[1] 黄爱文[1] 王秋实[1] 赵培冉[1] 梁双武[1] 

机构地区：[1]南方医科大学南方医院创伤骨科实验室,广州510515

出　　处：《中华外科杂志》2008年第16期1249-1252,共4页Chinese Journal of Surgery

基　　金：国家高技术发展计划（863计划）重大专项课题资助项目（2003AA205001）;国家自然科学基金资助项目（30600643）

摘　　要：目的比较单纯血管、神经束分别植入组织工程骨修复兔骨缺损对神经肽降钙素基因相关肽（calcitonin gene related peptide，CGRP）和神经肽Y（neuropeptide Y，NPY）表达影响。方法36只新西兰大白兔，随机平均分为3组：A组为组织工程骨＋股血管束植入，B组为组织工程骨＋感觉神经束植入，C组为单纯组织工程骨。每只动物均在右侧股骨制作长1．5cm的段缺性骨与骨膜缺损，钢板固定后分别用以上方法修复大段骨缺损。术后3、6、12个月行免疫组化染色检测修复骨段内的CGRP和NPY的表达，并采用图像分析软件行半定量分析。结果CGRP和NPY的表达量在A、B、C3组均随时间推移而增多（P=0．000），在3个月时A组较B组多（P=0．000），但在6个月和12个月时A组和B组的表达量无明显差异（P〉0．05）。A、B两组在3、6、12个月时的表达量均较C组多（P=0．000）。结论与单纯神经束植入组相比，单纯血管束植入组织工程骨修复兔骨缺损在术后3个月可以明显促进CGRP和NPY表达，但后期两组对神经肽表达的影响作用无明显差异。Objective To evaluate the different effect on the expression of Calcitonin gene related peptide （CGRP）and neuropeptide Y （NPY） between tissue engineered bone with vascular bundle graft in vivo and that with sensory nerve tract graft in vivo. Method Thirty-six healthy New Zealand rabbits were divided into 3 groups randomly and equally ： vascular bundle group （ A ）, sensory nerve tract group （ B ）, tissue-engineering group （C）. Group A segmental bone defect of 1.5 cm long was made at the right femur in each animal. After plate fixation, the defects were implanted respectively with the engineered bone prepared in the above-mentioned 3 methods. At 3, 6 and 12 months post-operatively, the distribution of CGRP and NPY in the new bone were detected by immunohistochemistry and analyzed semi-quantatively by image analysis software. Results CGRP and NPY immuno-histochemical results indicated their contents increased significantly in all 3 groups as time passed（P =0. 000）. Compared with group B, the contents of CGRP and NPY in group A significantly increased at 3 months （ P = 0. 000 ）, but there was no statistic difference between them at 6 or 12 months（P 〉0. 05）. The expression of CGRP and NPY in both group A and B were significantly more than that in group C at 3,6 or 12 months （P = 0. 000）. Conclusion Implantation of vascular bundle into tissue-engineered bone can significantly improve the CGRP and NPY contents at early 3 months comparing with Implantation of sensory tract into tissue-engineered bone, but the changes are not significant at 6 or 12 months pest-operatively.

关 键 词：组织工程 骨 血管束 感觉神经束 降钙素基因相关肽 神经肽Y 

分 类 号：R686[医药卫生—骨科学]