1～13岁健康儿童年龄相关的网织红细胞血红蛋白含量参考范围的测定  

Age related reference value for reticulocyte hemoglobin equivalent among children aged one to thirteen years

作　　者：王月芳[1] 杨惠[1] 王霞[1] 李娟[1] 陈岚[1] 王泓[1] 陈琪[1] 江咏梅[1] 

机构地区：[1]四川大学华西第二附属医院检验科,成都610041

出　　处：《中华检验医学杂志》2008年第7期767-770,共4页Chinese Journal of Laboratory Medicine

摘　　要：目的建立1～13岁健康儿童年龄相关的网织红细胞血红蛋白含量（RET-He）的参考范围。方法采用Sysmex XE-2100全自动血细胞分析仪检测424名健康儿童RET-He，于每日质控合格后检测临床标本，对所得数据极值进行处理；参考范围分别用第2．5百分位数（P2.5）和第97．5百分位数（P97.5）区间确定，并利用曲线拟合方法建立年龄相关的变化曲线函数。结果按性别分组，男、女RET-He中位数分别为31．30pg、31．80pg，经非参数两个独立样本检验，组间比较差异无统计学意义（Z=-2．762，P〉0．05），将男女2组数据合并，所建年龄相关的RET-He的参考范围最佳曲线方程如下：R2.5=-0．008X^3＋0．125X^2-0．178X＋26．456；P97.5=0．021X^2-0．184X＋34．670（X=年龄），帮分别为0．85、0．90，P均〈0．05，所建方程有统计学意义。结论健康儿童的RET-He含量有特殊性，与成人参考范围存在差异，建立健康儿童年龄相关的RET-He参考范围对贫血的诊断、鉴别诊断以及早期监测造血功能恢复的程度具有临床应用价值。Objective To establish age related reference value for the new reticulocyte hemoglobin equivalent （RET-He） parameter in healthy children aged one to thirteen years. Methods Reticulocyte hemoglobin equivalent was measured by Sysmex XE-2100 after completing daily internal quality control in 424 healthy children and the outlying observations was handled afterward. Age related reference value and distribution of RET-He level were described as the 2.5th and 97.5th centiles, and the equation regarding age related reference value was established through curve fitting methods. Results After grouping according to sex, the medians of boys and girls in RET-He were 31.30 pg,31.80 pg. Non-parametric test showed there was no differences between the boys and girls. When the data from two group was integrated together, the fitted equations of RET-He were： P2.5 = - 0. 008X^3 ＋ 0. 125X^2 - 0. 178X ＋ 26. 456; P97.5 = 0. 021X^2 - 0. 184X ＋ 34. 670（X refers to age） ; R^2 were 0. 85 and 0. 90,respectively（P 〈0. 05 ）. There was statistically significance for the above equations. Conclusions The reference value of RET-He in healthy children is different from those in adult. The equations can be used to produce age related reference value. It could be useful for anemia diagnosis, differential diagnosis and hematopoiesis screening at early stage.

关 键 词：红细胞指数 参考值 年龄因素 儿童 

分 类 号：R686[医药卫生—骨科学]