visfatin对大鼠肝脏胆固醇代谢及SREBP-2的调控作用  

Effects of visfatin on hepatic cholesterol metabolism and expression of SREBP-2 gene in rat

作　　者：李仁哲[1] 杨刚毅[2] 李伶[1] 孙勤[3] 刘建雷[1] 石绍川[1] 程玉兰[1] 

机构地区：[1]重庆医科大学检验系临床生化教研室和教育部实验诊断重点实验室,重庆400016 [2]重庆医科大学附属第二医院内分泌科,重庆400016 [3]四川省人民医院老年科,四川成都610072

出　　处：《第四军医大学学报》2008年第15期1382-1384,共3页Journal of the Fourth Military Medical University

基　　金：国家自然科学基金(30771037);重庆市教委科研基金(JK050304);美国NIH基金项目(R01HL073267;DK066003)

摘　　要：目的:探讨visfatin对血浆胆固醇代谢和肝胆固醇合成的影响.方法:将空载体转染对照组(NC组),大鼠visfa-tin基因真核表达质粒转染普通饲料组(NT组)和高脂组(HT)大鼠,并测定转染前后各组大鼠血浆胆固醇及肝脏HMG CoA还原酶和固醇调节元件结合蛋白2(SREBP2)mR-NA表达的变化.结果:visfatin质粒注射3 d后,NT和HT组大鼠血浆胆固醇均较注射前明显下降[(1.61±0.18)vs(1.25±0.09)mmol/L和(2.37±0.11)vs(1.74±0.12)mmol/L,均P<0.05];高密度脂蛋白胆固醇(HDL-C)也明显降低[(0.83±0.09)vs(0.56±0.09)mmol/L和(1.32±0.09)vs(0.91±0.04)mmol/L,P<0.05];NT和HT组大鼠SREBP2mRNA表达均明显高于NC组[(0.37±0.06),(0.36±0.07)vs(0.21±0.04),均P<0.05],NT组HMG CoA还原酶mRNA水平明显高于NC,HT组[(0.89±0.13)vs(0.37±0.07)和(0.55±0.16),均P<0.05],NC组与HT组无明显差异.结论:visfatin可能通过促进SREBP2和HMG CoA还原酶表达来调节体内胆固醇代谢.AIM： To investigate the effects of visfatin on hepatic cholesterol metabolism and the expression of SREBP-2 gene in rat. METHODS： The control plasmid pcDNA3.1 （ ± ） and recombinant plasmid pcDNA3. 1-visfatin were transfected into rats in control group（NC） ,normal diet group（NT） and high-fatty-diet group（ HIT）, respectively. The plasma cholesterol, hepatic HMG CoA reductase and sterol regulatory element binding protein 2 （SREBP2） mRNA were evaluated by RT-PCR before and after transfection. RESULTS： Plasma total cholesterol （TC） [ （ 1.61 ± 0. 18）vs（1.25 ±0.09 ）mmol/L and （2. 37 ±0. 11）vs （ 1.74 ± 0. 12） mmol/L,P 〈0.05 ] and high-density lipoprotein cholesterol（HDL-C） [ （0.83 ± 0.09） vs （0.56 ± 0. 09） mmol/L and （1.32±0.09） vs （0.91 ±0.04） mmol/L, P〈0.05]Of group NT and HT were significantly decreased after 3 d of pcDNA3.1- visfatin transfection; SREBP2 mRNA [ （ 0. 37 ± 0. 06 ） and （0.36 ±0.07） vs（0. 21±0.04） ,P 〈0.05] and HMG CoA reductase mRNA[ （0.89 ± 0.13 ） and （0.55 ± 0.16） vs （0.37 ± 0.07 ）, P 〈0.05 ] levels were significantly higher than those of group NC, and HMG CoA reductase mRNA level of group NT was higher than that of group HT, but there was no significant differ-ence between group NT and HT in SREBP2 mRNA levels. CONCLUSION： Visfatin regulates cholesterol metabolism by enhancing expression of SREBP2 and HMG COA reductase.

关 键 词：内脂素 胆固醇 HMGCoA还原酶 固醇调节元件结合蛋白2 

分 类 号：R587.1[医药卫生—内分泌]