糖皮质激素对大鼠胫骨松质骨密度和微结构的动态影响  

Dynamic effects of glucocorticoid on bone mineral density and microarchitecture： experiment with rats

作　　者：刘石平[1] 陈晶[1] 杨淑敏[1] 伍贤平[1] 廖二元[1] 盛志峰[1] 莫慧[1] 马育林[1] 章玉海[1] 刘唯[1] 金艳[1] 戴如春[1] 

机构地区：[1]中南大学湘雅二医院代谢内分泌研究所,长沙410011

出　　处：《中华医学杂志》2008年第32期2281-2284,共4页National Medical Journal of China

基　　金：湖南省科技厅计划基金资助项目（05SK3017）

摘　　要：目的应用显微CT研究糖皮质激素（GC）随时间延长对大鼠胫骨松质骨密度和微结构的影响。方法52只3．5个月龄雌性SD大鼠随机分为基线组10只、糖皮质激素造模组（GCT）22只和对照组20只。基线组10只实验开始时即处死，GCT组和对照组分别在实验开始后1周、9周各处死11只和10只，分别保存右侧胫骨，行显微CT扫描，并在扫描完成后，进行三维重建，获取骨小梁三维结构图像，最后进行松质骨定量分析。结果GCT组的体积骨密度（vBMD）、组织骨密度（tBMD）、骨体积分数（BVF）、骨小梁数量（Tb．N）、各向异性度（DA）和骨小梁连接密度（Conn．D）较对照组下降，9周时，改变更显著（均P〈0．05），而骨小梁厚度（Tb．Th）、骨小梁间隔（Tb．Sp）和结构模型指数（SMI）较对照组升高；GCT组随造模时间的延长，BVF、Conn．D、Tb．N和DA进一步下降，Tb．Th和Tb．Sp显著增加（均P〈0．05）。GCT组1周和9周时，Tb．Th分别为（0．076±0．020）mm和（0．086±0．026）mm，高于基线组（0．067±0．014）mm，也高于对照组1周（0．075±0．022）mm和9周（0．072±0．009）mm。结论GC可引起大鼠胫骨松质骨进行性骨量下降和骨微结构破坏，但残余的骨小梁有代偿性肥厚表现。Objective To investigate the Dynamic effects of glucocorticoid （ GC ） on bone mineral density and microarchitecture time-related changes of trabecular bone in bone mineral density （BMD） and microarchitecture in glucocorticoid -treated rats. Methods Fifty-two 3.5-month-old female SD rats were randomly divided into 3 groups. Ten rats were killed at the beginning of experiment with their right tibiae taken out as the baseline group; 22 rats underwent subcutaneous injection of methylprednisolone once daily （ GC-treated group）, and the other 20 rats underwent subcutaneous injection of normal saline once daily as control group. One and 9 weeks after the beginning of experiment 11 and 10 rats from GCT Group and control group each were killed with their right tibiae taken out. High resolution micro-CT was used to identify the densimetric and microarchitectural properties of the trabecula in the proximal metaphysic of tibia. Results Compared with the control group the values of volumetric BMD （ vBMD ）, tissue BMD （ tBMD ）, bone volume fraction （ BVF ）, trabecular number （ Tb. N ）, degree of anisotropy （ DA ）, and trabecular connectivity （Conn. D） in the trabecular bone at different time-points, of the GCT group all decreased; and the values in the ninth week were the lowest （ all P 〈 0. 05 ）. The values of trabecular thickness （ Tb. Th ）, trabecular separation （Tb. Sp）, and structure model index （SMI） at different time-points of the GCT group were higher than those of the control group. A time-related analysis within the GCT group showed there was a declination in BVF, Conn. D, Tb. N, and DA with administration time, but Tb. Th and Tb. Sp were increased significantly （ all P 〈 0. 05）. The mean values of Tb. Th in the first week and the ninth week of GCT Group were （ 0. 076 ± 0. 020 ） mm and （ 0. 086 ± 0. 026 ） mm respectively, both higher than the baseline value [ （0. 067 ± 0. 014） mm] and the values of the control group in the first and ninth week

关 键 词：胫骨 糖皮质激素 骨密度 

分 类 号：R686[医药卫生—骨科学]