11种非必需氨基酸对离体植物生长的胁迫作用  

Stress on the Growth of Plants in vitro of Non-essential Amino Acids

作　　者：杨靖[1] 孙海燕[1] 李友勇[1] 

机构地区：[1]河南科技学院,河南新乡453003

出　　处：《生物技术》2008年第4期72-74,共3页Biotechnology

基　　金：河南省自然科学基金项目资助(0511031100);河南科技学院重点科研项目资助

摘　　要：研究了11种非必需氨基酸对小麦、水稻等植物离体胚植株以及拟南芥、白菜等植物种子植株生长的影响，结果显示，L-半胱氨酸、L-酪氨酸和L-丝氨酸在浓度达到3mmol·L“时开始抑制植株生长，而L-谷氨酸、L-谷氨酰胺、L-甘氨酸和L-天冬氨酸4种氨基酸在5—7mmol·L^-1时才有轻微抑制作用；L-胱氨酸、L-天冬酰胺和L-丙氨酸3种氨基酸，在5．7mmol·L^-1内没有抑制作用。脯氨酸的3种异构体在9mmol·L^-1浓度下没有抑制作用发生，且还表现出有约1个生长级别（3．4cm左右）的生长促进作用。丝氨酸和丙氨酸的D-异构体在3mmol·L^-1浓度即有强烈的生长抑制作用。这些结果表明非必需氨基酸胁迫植物的途径有多态性，这些多态性对研究植物氨基酸代谢有重要意义。Embryos seedlings of wheat and rice, and seeds seedling of Chinese cabbage,arabidopsis et al being cultured in vitro in which 11 kinds of non - essential amino acids were added at the different concentration, th3 results show that L- Cys, L - Tyr and L - Ser could inhibit growth of seedling at the concentration of 3 mmol· L-1, and for L - Gin, L - Gin, L - Gly and L - Asp, the concentration of inhibiting growth increased up to 5 - 7 mmol·L-1 , and for L- Cystine, L- Asp and L- Ma, seedling＇s growth were not inhibited at the same concentration of 5 - 7 mmol· L-1 . All of the three kinds of isomers of proline did not inhibit, but promoted seedling＇s growth in some way up to the concentration of 9 mmol· L-1 . D - isomers of both Serline and Alanine suppressed growth severity at lower concentration, 1 - 3mmol· L- 1. These results suggested that the way that non- essential amino acids stress plants have polymorphism that have important role to research plant amino acids metabolism.

关 键 词：非必需氨基酸 胁迫 离体植物 小麦 拟南芥 

分 类 号：Q813.1[生物学—生物工程]