实验性变应性鼻炎最轻持续炎性反应对豚鼠鼻黏膜的影响  

Effects of minimal persistent inflammation on nasal mucosa of experimental allergic rhinitis in guinea pigs

作　　者：雷霏[1] 崔亚南[2] 姜云鹏[3] 孔红[1] 朱冬冬[1] 董震[1] 

机构地区：[1]吉林大学中日联谊医院耳鼻咽喉头颈外科, 长春130033 [2]吉林大学中只联谊医院中心研究室,长春130033 [3]吉林大学基础医学院病理生物学教育部重点实验室

出　　处：《中华耳鼻咽喉头颈外科杂志》2008年第7期499-503,共5页Chinese Journal of Otorhinolaryngology Head and Neck Surgery

摘　　要：目的建立豚鼠实验性变应性鼻炎最轻持续炎性反应（minimal persistent inflammation，MPI）模型，观察长期处于MPI状态下鼻黏膜的改变，初步探讨这一过程中转化生长因子β1（transforming growth factor—β1，TGF—β1）和基质金属蛋白酶9（matrix metalloproteinases-9，MMP-9）表达的变化。方法健康雄性Hartley系豚鼠30只，随机分为MPI模型组和对照组，每组15只。首先以含卵清蛋白（ovoalbumin，OVA）和氢氧化铝凝胶的生理盐水混合液行基础致敏和强化致敏，再以低浓度OVA长期鼻腔激发，建立豚鼠变应性鼻炎MPI模型。通过阿辛蓝-过碘酸-希夫（alcian blue—periodic acid-Schiff，AB—PAS）染色和Masson三色胶原染色（Masson’s trichrome，MT），分别检测鼻黏膜上皮内杯状细胞的数量以及基底膜内胶原的含量；采用双重免疫荧光法标记TGF—β1和MMP-9，激光共聚焦显微镜下观察两种因子在鼻黏膜内的分布及表达情况。结果与对照组相比，MPI模型组豚鼠鼻黏膜内杯状细胞数量明显增多，差异具有统计学意义（t=13．720，P〈0．05）；基底膜明显增厚，胶原含量增多（t=4．542，P〈0．05）。对照组豚鼠鼻黏膜内，TGF—β1几乎没有表达，MMP-9只在纤毛上皮细胞内有轻微的表达。MPI模型组豚鼠鼻黏膜内，两种因子均有表达。TGF—β1主要表达于黏膜上皮细胞及固有层内浸润的炎细胞胞质和细胞外基质；MMP-9主要表达于黏膜上皮细胞及固有层内浸润的炎细胞胞质中，其在黏膜上皮细胞内的表达强度高于对照组（t=25．218，P〈0．05）。结论长期处于变应性鼻炎MPI状态下，豚鼠鼻黏膜内出现了具有组织重塑特征的改变，TGF—β1和MMP-9可能在这一过程中发挥作刚。Objective To establish an animal model of minimal persistent inflammation （MPI） of allergic rhinitis in guinea pigs and to investigate the changes of nasal mucosa. The expression of transforming growth faetor-β1 （TGF-β1） and matrix metalloproteinases-9 （MMP-9） were discussed. Methods Thirty male Hartley guinea pigs were randomly divided into two groups： MPI model group and control group randomly, with fifteen animals in each group. Guinea pigs from MPI model group were sensitized intraperitoneally by injection of suspension of ovoalbumin （OVA） and aluminum hydroxide in 0. 9% physiological saline. Then, repeated local booster sensitization with low concentration of OVA suspension into the nasal cavity was performed to establish MPI models. Alcian blue-periodic acid-Schiff （AB-PAS） staining and Masson＇s trichrome （MT） staining were used to determine the number of goblet cells and collagen deposition within the basement membrane of epithelium. The expression and distribution of TGF-β1 and MMP-9 in nasal mucosa were estimated by double immunofluorescence under a confocal laser scan microscopy system. Results Compared with the control group, the increased goblet cells （t = 13. 720, P 〈 0. 05） in nasal epithelium together with the increased collagen fibrils （ t = 4. 542, P 〈 0. 05 ） within the basement membrane of epithelium were observed in the MPI model group. There was nearly no expression of TGF-β1 in the control group and the expression of MMP-9 was only found in the epithelium cell. In contrast,there was significantly higher expression of TGF-β1 and MMP-9 （ t = 25. 218, P 〈 0. 05 ） in nasal mucosa of MPI model group than that in control group. TGF-β1 mainly expressed in the epithelium cell, the infiltrated inflammatory cell and extracellular matrix, while MMP-9 expressed in the epithelium cell and the infiltrated inflammatory cell. Conclusions Long time MPI in allergic rhinitis resulted in some changes of tissue remodeling in nasal mucosa. TGF-β1 and MMP-9 may

关 键 词：鼻炎 变应性 季节性 模型 动物 转化生长因子B 明胶酶B 

分 类 号：R686[医药卫生—骨科学]