Smad2/3蛋白及其活化形式在人肾脏中的表达和定位  

EXPRESSION AND LOCALIZATION OF SMAD2/3 AND P-SMAD2/3 IN HUMAN KIDNEY

作　　者：肖泽均[1] 贾永忠[2] 董静霞[3] 迟晓春[3] 祁丽花[3] 张承英[2] 徐健[3] 

机构地区：[1]中国协和医科大学中国医学科学院肿瘤医院泌尿外科,北京100021 [2]武警总医院泌尿外科,武警总医院肾内科北京100039 [3]北京大学医学部基础医学院解剖与组织胚胎学系,北京100083

出　　处：《中国组织化学与细胞化学杂志》2008年第4期345-348,共4页Chinese Journal of Histochemistry and Cytochemistry

基　　金：北京大学医学部985基金(61220600062)

摘　　要：目的研究Smad2和Smad3蛋白及其活化形式P-Smad2、P-Smad3在人正常肾脏组织中的表达、定位及其意义。方法采用免疫组织化学技术(SP法)检测20例人肾脏中Smad2、Smad3蛋白及P-Smad2、P-Smad3的表达和定位。结果Smad2、Smad3在肾小管、肾小球和集合小管中广泛表达,主要定位于细胞质,其中远端小管曲部呈强阳性;P-Smad2、p-Smad3也在肾脏皮、髓质中广泛分布,主要定位于细胞核,远端小管更多见。结论Smad2、Smad3在正常肾脏中有着活跃的功能。Objective To investigate the expression and localization of Smad2/3 and phosphorylated-Smad2/3 in human kidney. Methods Immunohistochemistry was used to analyze 20 specimens of human renal tissue. Results Smad2 and Smad3 proteins were widely expressed in renal tubules and corpuscles, and mainly localized within the cytoplasm of the epithelia lining the renal tubules and collecting ducts, especially remarkable in the epithelia of distal convoluted tubule. P-Smad2 and p-Smad3 were mainly distributed in the nuclei in renal cortex and modulla, with more frequent localization in distal convoluted tubules. Conclusion Smad2 and Smad3 play active roles in normal human renal function.

关 键 词：SMAD 肾脏 免疫组化 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学]