大鼠电流损伤后心肌PEG-3表达的法医学研究  

The study for expression of PEG-3 in rats’ cardiac muscle after electrical injury by the method of immunohistochemistry

作　　者：郝建文[1] 万立华[1] 李万军[2] 

机构地区：[1]重庆医科大学基础医学院法医学教研室,重庆400016 [2]汉中市3201医院病理科,汉中723000

出　　处：《重庆医科大学学报》2008年第8期947-950,共4页Journal of Chongqing Medical University

摘　　要：目的:探讨电击伤和电击死后心肌中PEG-3表达的时间相关性以及其能否用于生前电击和死后电击的鉴别。方法:建立大鼠电流损伤的模型。电击伤组于电击后0h、3h、6h、12h、24h、3d、6d、12d断颈处死取材;电击死组分别于死后0h、1h、3h、6h、12h取材;死后电击组分别于断颈处死后的0h、0.5h、1h、3h电击后取材;阴性对照组直接断颈处死后分别于0h、0.5h、1h、3h、6h、12h取材,取材的部位均为左心室前壁中央部,取材后采用免疫组化染色法检测所有大鼠心肌PEG-3的表达情况,其结果经图象分析处理后进行统计分析。结果:电击伤(死)组在电损伤早期PEG-3即表达增加,且不同时间的表达有一定的规律,故可用于推断心肌电击伤(死)的时间。而在死后电击及阴性对照组只有弱表达,根据这点可以将死后电击和电击致死进行区分。结论:心肌电损伤后PEG-3的表达有一定的时间相关性,可以用于对电击伤(死)的诊断;心肌电损伤后PEG-3的表达情况可以鉴别生前电击和死后电击。Objective：To study the expression of PEG-3 in cardiac muscle after electrical injury in order to find the time dependablity and the discrimination for the antemortem electrified and postmotorm electrified. Metheds：The animals were derided into two groups：the negative group and the experimental group.The experimental group were derided into three groups again：the electrical injury group,the electrouction group and the group of electrical shock after death. The electrical injury group is for 0h,3h,6h,12h,24h,3d,6d,12d,the electrouetion group is for 0h,1h,3h,6h,12h,and the group of electrical shock after death is for 0h,0.5h,1h,3h,the control group is for 0h,0.5h,1h,3h,6h,12h. After all these we should get the cardiac tissue at the propotional time,and then dye it hy the method of HE and immunohistoehemistry. Then we can gain the conclusion through the method of semiquantitative analysis. Results：The expression of PEG-3 increased in heart injury early, it may be a useful molecular index to diagnose heart injury induced by electric force. In additional,the rule of PEG-3 expression was changed following time,which may help to estimate the injury interval in forensic El field. We can also discriminate the the electrouction group and the postmotorm electrified group,because there are only extremely tenuous positive staining for the group of electrical shock after death. Conclusion：PEG-3 can diagnose the electrical injury and discriminate the electrical injury,weather it formed at antemortem or postmortem.

关 键 词：心肌 电流损伤 PEG-3 图像分析 

分 类 号：R89[医药卫生—法医学]