苏云金芽胞杆菌酰基高丝氨酸内酯酶基因的克隆及分析  

Cloning and sequence analysis of AHL-lactonase gene from Bacillus thuringiensis

作　　者：黄天培[1] 潘洁茹[1] 姚帆[1] 黄张敏[1] 

机构地区：[1]福建农林大学生物农药与化学生物学教育部重点实验室,福建福州350002

出　　处：《福建农林大学学报（自然科学版）》2008年第4期374-378,共5页Journal of Fujian Agriculture and Forestry University:Natural Science Edition

基　　金：国家863计划(2006AA10A212);国家自然科学基金(30571257);教育部博士点基金(20060389011);福建省青年科技人才创新项目(2006F3016);福建省教育厅科技计划项目(JA07066);福建省重点引智项目(SZ2007035)资助

摘　　要：利用pMD18-T克隆载体从苏云金芽胞杆菌菌株WB12中克隆了酰基高丝氨酸内酯酶基因(AHL-lactonase,aiiA).测序结果表明,该基因(GenBank登录号为DQ000642)由753个碱基组成,编码含有250个氨基酸残基的蛋白质.推测该蛋白质分子质量为28 ku,等电点为4.2,不含信号肽序列.利用DNAMAN软件构建同源关系树.结果表明,该蛋白与已报道具有减弱欧文氏菌胡萝卜亚种致病力的11个A iiA蛋白酶氨基酸序列总相似性为81.2%,并含有相同的氨基酸序列保守区.核苷酸序列的BLAST分析结果表明,与之同源性较高的基因均为蜡质芽孢杆菌组aiiA基因(86%-99%).A novel AHL-lactonase gene （aliA） was cloned from Bacillus thuringiensis WB12 by PCR with pMD18-T vector and sequenced. The nuclcotide sequence has been registered in GenBank with accession number DQ000642. It consists of 753 bases which encxxtes a polypeptide of 250 amino acids residues with a calculated molecular weight of 28 ku and an isoelectric point value of 4.2. No signal peptide was found in WB12 AliA. On the basis of the multiple alignment of amino acid sequences of this Alia and other 10 AliA, which are capable of attenuating the plant pathogenicity of Erwinia carotovora var. carotovora, a homology tree was con- structed using DNAMAN. The result showed that the amino acid sequences of the proteins were 81.2% identical overall and share same conserved regions. BLAST analysis of its nucleotide sequence showed 86% - 99% identity with those of the aliA genes from B. cereus group.

关 键 词：苏云金芽胞杆菌 酰基高丝氨酸内酯酶基因 克隆 序列分析 

分 类 号：S476[农业科学—农业昆虫与害虫防治] Q785[农业科学—植物保护]