不同品系小鼠主动脉弓缩窄压力负荷模型的比较  被引量:4

Comparison of Heart Function Changes by Transverse Aortic Constriction in Different Strain Mice

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作  者:李晓梅[1] 马依彤[1] 杨毅宁[1] 刘芬[1] 黄莺[1] 韩伟[1] 高晓明[2] 张健发[1] 

机构地区:[1]新疆医科大学第一附属医院心脏中心,乌鲁木齐830054 [2]Baker心脏研究所实验心脏病学研究室

出  处:《实验动物与比较医学》2008年第4期209-214,共6页Laboratory Animal and Comparative Medicine

基  金:新疆维吾尔自治区高技术研究发展计划项目(XJKTJ200511110)

摘  要:目的比较两种不同品系小鼠建立主动脉弓缩窄压力负荷模型后心脏形态及功能变化的差异。方法将雄性KM小鼠及C57BL/6小鼠各一组随机分为术前(n=10)、假手术组(n=10)及主动脉弓缩窄(TAC)组(n=30)。术前及术后8周应用高频心脏超声、血流动力学、心脏病理组织学方法进行检测,并进行死亡原因观察。结果(1)KM小鼠及C57BL/6小鼠缩窄组围手术成功率分别为80%及87%;8周存活率分别为67%及77%(。2)与各假手术组比较,缩窄术后8周KM及C57BL/6小鼠TAC组心脏超声指标左心室收缩期、舒张期前壁厚度(Awsth;Awdth)、左心室收缩期、舒张期后壁厚度(Pwsth;Pwdth)、左室重量指数(LVMI)、动脉收缩压(SBP)、动脉舒张压(DBP)、左心室收缩压(LVSP)、左心室舒张末压(LVEDP)、左心室压力上升和下降最大速率(dp/dtmax;dp/dtmin)及病理解剖指标心脏重量指数(H w/Bw)、左心室重量(LVw)、左心室重量指数(LV w/Bw)均显著增加(均P<0.05),显示左心室肥厚;KM缩窄组左心室收缩末期、舒张末期内径(LVESd;LVEDd)、左心室舒张末期外径(EXLVDd)较其假手术组显著增加(均P<0.05);与C57BL/6缩窄组比较,术后8周KM缩窄组LVESd、LVEDd、EXLVDd、LVw、肺脏重量指数(Lungw/Bw)显著增加(均P<0.05)。结论KM小鼠主动脉弓缩窄动物模型与C57BL/6小鼠实验结果相似,均产生左室肥厚,但KM小鼠较早出现离心性肥厚趋势。Objective To compare difference of the function of the hypertrophic hearts induced by transverse aortic constriction(TAC) in different strain mouse with microsurgery. Methods KM and C57BL/6 mice randomly divided into three groups: before operation (BO)(n=10), sham-operation (SH) (n=10) and transverse aortic constriction(TAC)(n=30). Echocardiographic assessments, hemodynamic determineation, organ weight measurement and histological examination were performed before and at 8 weeks after surgery, and observed the cause of death. Results The successful rate of KM and C57BL/6 TAC groups was 80% and 87%. The survival rate of two TAC groups was 67% and 77% after 8 week; Compared respectively with SH group, at 8 week after surgery, anterior wall thickness at systole and diastole (Awsth; Awdth), posterior wall thickness at systole and diastole (Pwsth; Pwdth), LV mass index (LVMI), systolic blood pressure(SBP), diastolic blood pressure (DBP), left ventricular systolic pressure (LVSP), left ventricular end-diastolic pressure (LVEDP), rate of rise of left ventricular pressure (± dp/dtmax), the heart weight-to-body weight ratio (Hw/Bw), left ventricular Weight(LVw), the LV weight-to-body weight ratio (LVw/Bw) of KM and C57BL/6 TAC groups increased significantly(P〈0.05). Compared with KM SH group, the left ventricular end-systolic dimension (LVESD), Ventricular end-diastolic dimension (LVEDd), external left ventricular diastolic diameter (EXLVDd) ofKM TAC group increased markedly (P〈0.05). Compared with C57BL/6 TAC group, LVESD, LVEDd, EXLVDd, LVw, the Lung weight-to-body weight ratio(Lungw/Bw) of KM TAC group increased markedly (P〈0.05).Conclusion KM mice can establish a TAC model that induces the myocardium of LV from hypertrophy to heart failure. The result is as the same as C57BL/6 mice's. KM mice shows the tendency of eccentric hypertrophy early.

关 键 词:主动脉弓缩窄 左室肥厚 小鼠 

分 类 号:Q95-33[生物学—动物学]

 

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