皖南山区猕猴种群的形态特征与微卫星遗传多样性初步分析  

Morphological Characters and Genetic Polymorphism Analysis by Microsatellite Loci in Rhesus Monkey Stock from Wannan Mountains

作　　者：张卉[1] 李进华[1] 赵健元[1] 汪腾海 

机构地区：[1]安徽大学生命科学学院安徽省生态工程与生物技术重点实验室 [2]安徽省实验猕猴中心,黄山245604

出　　处：《实验动物与比较医学》2008年第4期225-229,共5页Laboratory Animal and Comparative Medicine

基　　金：国家自然科学基金(30570237);安徽省教育厅科技创新团队计划项目(TD200703);安徽省优秀青年基金(040443070);安徽省教育厅自然科学基金(2005KJ021ZD);安徽省人才开发基金;安徽省科技厅科技条件专项;安徽大学学术创新团队项目资助

摘　　要：目的为建立封闭群实验猕猴,对皖南山区猕猴种群的遗传背景进行了初步调查。方法活体测量了18只猕猴的形态特征变量,并使用微卫星标记对33只猕猴进行了遗传多样性分析。结果体重变量具有显著的性别差异,躯干变量具有显著的年龄差异。同时从12个微卫星标记中筛选出9个具多态性标记,在猕猴群中平均检测到8个等位基因,群体杂合度H=0.825±0.106,多态信息含量PIC=0.788±0.121。结论皖南山区猕猴种群的形态特征体现了福建亚种的特性,在微卫星水平上表现出较高的遗传多样性,适合进行引种并建立猕猴的封闭群。Objective In order to establish the closed colony, the heredity background of the rhesus monkeys from Anhui stock were investigated. Method Several morphological variables of 18 individual monkeys from Anhui Wannan Mountains were measured, and the genetic polymorphism of 33 monkeys was analyzed by microsatellite loci. Result The results of t-test showed that there were evident differences in weight between male and female and that in trunk length between adult and subadult. At the same time, nine polymorphic microsatellite loci were selected from twelve loci. The mean number of alleles of Anhui rhesus monkey stock detected was 8. The heterozygosity value was 0.825±0.106, and the polymorphism information content value was 0.788±0.121. Conclusion Morphological traits of Anhui rhesus monkey stock embodied the feature ofM.mulatta littoralis. And genetic diversity of the group is high on the microsatellite level. So we can screen out the original seed from Anhui rhesus monkey stock and establish the closed colony.

关 键 词：猕猴种群 形态特征 微卫星位点 遗传多样性 

分 类 号：Q953[生物学—动物学]