大鼠附睾脂肪间充质干细胞的分离培养和鉴定  

Isolation,culture and identification of adipose-derived stem cell in rats

作　　者：刘梦雪[1] 乔虹[1] 姜玉玲[1] 李强[1] 孙玉倩[1] 张巾超[1] 

机构地区：[1]哈尔滨医科大学第二临床医学院内分泌-代谢病科,黑龙江哈尔滨150081

出　　处：《哈尔滨医科大学学报》2008年第4期338-340,共3页Journal of Harbin Medical University

基　　金：黑龙江省自然科学基金(D200646);黑龙江省卫生厅医学科研课题(2005-114);黑龙江省研究生创新科研项目(YJSCX2007-0203HLJ)

摘　　要：目的成功体外分离培养大鼠附睾脂肪间充质干细胞(adipose-derived stem cell,ADSC),为组织工程提供种子细胞。方法应用胶原酶消化法分离大鼠ADSC,进行体外培养、连续传代,并观察细胞的形态变化;细胞计数绘制生长曲线;用MTT比色法测定细胞增殖活性;用流式细胞仪检测细胞周期;采用细胞免疫荧光化学方法检测表面标记。结果ADSC体外培养生长状况良好,呈均一的成纤维细胞样,增殖活跃,传代稳定,Vimentin、CD105表达阳性,α-SMA、CD31不表达。结论大鼠ADSC生长稳定,增殖能力活跃,便捷的分离培养方法能为其在组织工程中的应用奠定基础。Objective To obtain adipose-derived stem cell（ADSC） of rats. Methods ADSCs of Wistar rats were digested with collagenase type I solution. The growth curve of ADSCs was checked by cell counting;The cell proliferation was tested by MTT method;The molecular markers of ADSCs were detected with immunofluorescence. Results The morphology of ADSCs was identical to fibroblast-like and the cells showed active proliferative ability. Vimentin and CD105 antigens could be detected,but CD31 and α-SMA antigens were negative. Conclusion ADSCs can be used as seed cells for tissue engineering due to the simple isolation and stable,active growth of ADSCs.

关 键 词：脂肪间充质干细胞 胶原酶 分离 培养 细胞鉴定 

分 类 号：R329-33[医药卫生—人体解剖和组织胚胎学]