血竭素高氯酸盐防治糖尿病肾病小鼠肾损伤的作用及机制的研究  

Effect and mechanism of dracorhodin perchlorate on preventing and threating renal injury in diabetic nephropathy mice

作　　者：张丽晓[1] 刘建国[1] 王全胜[1] 汪永辉[1] 张彬[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院中医科,湖北武汉430022

出　　处：《医学研究生学报》2008年第8期800-804,共5页Journal of Medical Postgraduates

基　　金：国家自然科学基金资助项目(批准号:30600810)

摘　　要：目的:探讨血竭素高氯酸盐对糖尿病肾病(DN)所致肾损伤的防治作用。方法:用链脲佐菌素(STZ)单次腹腔注射的方法建立DN小鼠模型。设血竭素高氯酸盐20、10和5 mg/(kg.d)3个剂量治疗组,将血竭素高氯酸盐溶于等渗盐水中灌胃,以胰岛素治疗为对照组,同时设DN模型组及正常对照组。治疗4周后检测小鼠肾质量指数、肾小球细胞外基质(ECM)、24 h尿蛋白定量、血清肌酐清除率。用RT-PCR法检测血清和糖皮质激素诱导的蛋白激酶1(SGK1)、转化生长因子-β1(TGF-β1)及纤连蛋白(FN)的mRNA表达。结果:不同剂量血竭素高氯酸盐治疗组对SGK1、TGF-β1及FN的mRNA表达均有一定的抑制作用,其表达水平低于糖尿病(DM)模型组,其中血竭素高氯酸盐20和10 mg/kg组较正常对照组低(P<0.05)。血竭素高氯酸盐治疗组的肾质量指数、血清肌酐清除率、24 h尿蛋白定量及ECM均较DN模型组下降(P<0.05)。结论:血竭素高氯酸盐能防治小鼠DN的肾损伤,其机制可能与下调肾皮质TGF-β1、SGK1及FN的mRNA水平有关。Objective ：To study the effect of dracorhodin perchlorate on preventing and treating renal injury in diabetic nephropathy（DN）. Methods：8 weeks old C57BL/6 male mice were randomly divided into six groups： normal control group, DN model group, insulin control group （DN mice were administered by insulin）, dracorhodin perchlorate groups （ DN mice were administered of dracorhodin perchlorate at 20, 10, and 5 mg/（kg·d）. DN mice was induced by streptozotocin（STZ） 150 mg/（kg · d） peritoneal injection. The mice were sacrificed at the end of the 4^th week. The metabolic data were measured at 4^th week. The mRNA expression of Serum and glucocorticoid induced kinase 1 （ SGK1 ）, transforming growth factor-β1 （TGF-β1） and fibronectin （FN） were detected by RT-PCR. Renal function was evaluated by serum creatinine clear rate. Result; Dracorhodin perchlorate and insulin can reduce significantly the expression of SGK1, TGF-β1, FN and extracellular matrix （ECM） in kidney, and decrease significantly GFR （ glomerular filtration rate ） and proteinuria, and the reduction is dose-dependent on dracorhodin perchlorate treatment. Conclusion：Dracorhodin perchlorate can prevent and treat renal injury in diabetic nephropathy mice, and the mechanism may be related with the decrease of SGK1 ,TGF-β1 and FN mRNA in renal cortex.

关 键 词：血竭素高氯酸盐 糖尿病肾病 血清和糖皮质激素诱导的蛋白激酶1 转化生长因子-Β1 纤连蛋白 

分 类 号：R692[医药卫生—泌尿科学]