1,25二羟基维生素D_3对大鼠脾CD4^+CD25^+调节性T细胞及Foxp3基因表达的影响  

Effect of 1,25(OH)_2D_3 on CD4^+CD25^+ regulatory T cells and transcription factor Foxp3 in rat spleen

作　　者：李培[1] 祁晓平[1] 黎介寿[1] 

机构地区：[1]南京军区南京总医院(南京大学医学院临床学院)解放军普通外科研究所,江苏南京210002

出　　处：《医学研究生学报》2008年第8期805-808,共4页Journal of Medical Postgraduates

基　　金：国家重点基础研究发展规划基金资助项目(批准号:2003CB515502)

摘　　要：目的:探究1,25二羟基维生素D3[1,25(OH)2D3]对大鼠脾CD4+CD25+调节性T细胞(Treg)及其特异性转录因子Foxp3基因表达的影响。方法:近交系雄性Lewis大鼠,随机分成对照组和3个剂量的VitD3组:0.125μg组,0.25μg组和1μg组,每组30只;灌胃给药,3次/周,共2周后对照组给予赋形剂;各组随机抽取20只大鼠,于第15 d腹腔注射脂多糖(LPS)(10 mg/kg),随机选择其中10只于6 h后切取脾,其余10只观察注射LPS后96 h大鼠的病死率,同时留取未注射LPS大鼠的脾;流式细胞仪检测脾CD4+CD25+Treg数量变化,RT-PCR检测脾Foxp3 mRNA表达。结果:1,25(OH)2D3明显上调大鼠脾CD4+CD25+Treg的数量及特异性转录因子Foxp3mRNA的表达;1,25(OH)2D3能保护大鼠抵抗LPS的攻击。结论:1,25(OH)2D3对大鼠脾CD4+CD25+调节性T细胞及特异性转录因子Foxp3 mRNA的表达有显著影响。1,25(OH)2D3保护大鼠抵抗LPS攻击可能与其促进CD4+CD25+Treg的发育和功能有关。Objective： To investigate the effect of 1,25 （OH）2D3 on CD4^＋CD25^＋ regulatory T cells and the specific transcription factor Foxp3 in the rat spleen. Methods：Three groups of Lewis rats were fed with 1,25 （OH）2D3 at the dose of 0. 125 μg, 0.25 μg and 1 μg per day respectively for 14 days, isotonic Na chloride was given at the same dose to a control group, and then LPS （10 mg/kg） was administered intraperitoneally to the 3 experimental groups and half of the control rats on the 15th day. The animals were sacrificed 6 hours later, the percentage of CD4^＋CD25^＋ regulatory T cells in lymphomonocytes of the spleen was analyzed by flow cytometry and the expression of Foxp3 mRNA of the spleen was detected by real time RT-PCR. Results ： 1,25 （OH） 2 D3 obviously increased the percentage of CD4^＋CD25^＋ regulatory T cells and up-regulated the expression of Foxp3 mRNA in the rat spleen. Conclusion： 1,25 （OH）2 D3, with a great influence on CD4^＋CD25^＋ regulatory T cells and the specific transcription factor Foxp3, may enhance the development and function of CD4^＋CD25^＋ regulatory T cells either directly or indirectly.

关 键 词：1 25二羟基维生素D3 CD4^+CD25^+调节性T细胞 FOXP3 

分 类 号：Q786[生物学—分子生物学]