微创大鼠体外循环模型的建立  

A minimally invasive model of cardiopulmonary bypass in rats

作　　者：沈中华[1] 董国华[1] 黄海荣[1] 吴海卫[1] 朱家全[2] 景华[1] 

机构地区：[1]第二军医大学南京临床医学院(南京军区南京总医院)心胸外科,江苏南京210002 [2]上海交通大学医学院附属上海新华医院心胸外科,上海200092

出　　处：《医学研究生学报》2008年第8期809-812,共4页Journal of Medical Postgraduates

基　　金：江苏省自然科学基金青年科技创新人才项目(批准号:BK2005431)

摘　　要：目的:建立微创大鼠体外循环模型,为研究体外循环术后病理生理变化和评价治疗策略提供可靠、经济的实验平台。方法:20只成年SD大鼠,采用颈静脉、尾动脉、股动脉插管建立体外循环通路,在100 ml/(kg.min)流量下维持转流60 min,监测心率、血压、血气及电解质结果分析。结果:20只大鼠全部穿刺成功,在设定条件下完成体外循环,术后存活均>1周。结论:该模型具有经济、简单、微创、体外循环参数设置接近临床、模型成功率高、术后生存期长等优点,可作为研究体外循环后病理生理变化和评价治疗策略的可靠工具。Objective： To develop a minimally invasive model of eardiopulmonary bypass （ CPB ） in rats. Methods： Twenty adult SD rats were anesthetized, and the external jugular vein, the tail artery and the femoral artery were cannulated to establish CPB, which maintained at the flow rate of 100 mL/（ kg · min） for 60 min, while the heart rate, blood pressure, blood gas and electrolytes were monitored and analyzed. Results： All the animals successfully underwent cannulation and CPB and survived more than 1 week after the procedure. Conclusion： The CPB procedure, minimally invasive, easy to perform and with a high rate of survival, can be used as an effective tool for the study of the pathophysiologic changes and the evaluation of the therapeutic strategies associated with CPB.

关 键 词：体外循环 动物模型 

分 类 号：R331[医药卫生—人体生理学]