应用蛋白质芯片检测骨性关节炎患者基质金属蛋白酶-13的初步研究  

The research of detecting the MMP-13 in the synovia and serum of arthritic by protein array

作　　者：班吉鹤[1] 周利武[1] 毛广平[1] 郭亭[1] 唐祖明[2] 赵建宁[1] 

机构地区：[1]南京大学医学院临床学院(南京军区南京总医院)骨科,江苏南京210002 [2]东南大学生物医学工程系分子与生物电子学国家重点实验室,江苏南京210021

出　　处：《医学研究生学报》2008年第8期836-838,844,I0002,共5页Journal of Medical Postgraduates

基　　金：江苏省自然科学基金面上项目资助(批准号:BK2005085)

摘　　要：目的:探讨应用蛋白质芯片技术检测骨性关节炎(OA)患者关节液和血清中基质金属蛋白酶-13(MMP-13)的方法。方法:应用低密度蛋白质芯片方法同时检测32例OA患者关节液和血清中MMP-13表达水平,24例正常关节作为对照组,进行蛋白质芯片荧光强度的对比研究。结果:OA患者关节液和血清中的MMP-13在蛋白质芯片上的荧光强度明显高于对照组关节液的荧光强度(P<0.001)。OA患者关节液中MMP-13在蛋白质芯片上的荧光强度明显高于其血清的荧光强度(P<0.001)。结论:OA患者关节液和血清中MMP-13表达明显高于对照组。应用低密度的蛋白质芯片技术检测OA患者关节液及血清中MMP-13的水平具有一定临床应用价值。Objective： To investigate the expression of Matrix metalloproteinase-13 （MMP-13） in the synovia and serum of arthritis by protein array. Methods ： Expression of MMP-13 in the synovia and serum of 27 osteoarthritis（OA） patients and 17 health adults were measured by the low-density protein array, and the fluorescence intensity was compared immediately. Results： The MMP-13 ＇ s fluorescence intensity of the OA patients＇ synovia on the protein array were significantly higher than health group（P 〈 0.001 ） ; The MMP-13＇ s fluorescence intensity of the OA patients＇ serum on the protein array were significantly higher than health （P 〈0.001 ） ;The MMP-13＇ s fluorescence intensity of synovia on the protein array were significantly higher than the serum （P 〈 0.001 ）. Conclusion： The expression of MMP-13 in the synovia and serum of arthritis were significantly higher than the normal, the low-density protein array can be used to detect the level of the synovia and serum.

关 键 词：蛋白质芯片 骨性关节炎 基质金属蛋白酶-13 

分 类 号：R684.3[医药卫生—骨科学]