一株油藏嗜热厌氧杆菌的分离、鉴定及代谢产物特征  

Isolation and characterization of Thermoanaerobacter mathranii SC-2 from oil-field water

作　　者：黎霞[1] 承磊[1] 汪卫东[2] 邓宇[1] 尹小波[1] 张辉[1] 

机构地区：[1]农业部沼气科学研究所,成都610041 [2]中国石化胜利油田采油工艺研究院,东营257000

出　　处：《微生物学报》2008年第8期995-1000,共6页Acta Microbiologica Sinica

基　　金：国家自然科技资源平台项目(2005DKA21201);中国石化集团重点基础研究项目(P06071-3)~~

摘　　要：【目的】了解油藏环境中细菌的生理生化特性及代谢产物。【方法】采用Hungate厌氧操作技术从胜利油田罗801区块油层采出水中分离到一株厌氧杆菌SC-2。采用生理生化鉴定结合16SrDNA序列的系统发育学分析确定该菌株的系统发育地位,用气相色谱分析其代谢产物。【结果】菌株SC-2为严格厌氧的革兰氏阴性杆菌,菌体大小为0.38μm×1.7μm～3.9μm,单生、成对或成串生长,产端生芽孢。温度生长范围40℃～75℃(最适温度70℃);pH范围5.5～9.5(最适pH6.5);NaCl浓度范围0%～5%(最适NaCl浓度0%)。能够利用葡萄糖、麦芽糖、甘露糖、木糖等多种碳水化合物,发酵葡萄糖的产物是乙醇、乙酸、丙酸、H2、CO2及少量的乳酸。菌株SC-2的(G+C)mol%含量为30.8%,与Thermoanaerobacter mathranii subsp.mathranii的16S rDNA序列相似性为99.85%。菌株利用葡萄糖产乙酸、乙醇的最佳初始pH为8.0;酵母粉能刺激生长并显著提高发酵葡萄糖的产酸、产醇率;培养基中添加4%(V/V)的乙醇能明显抑制菌体生长。【结论】菌株SC-2是从特殊生境(油层采出水)中分离到的一株嗜热、耐盐的厌氧菌,其发酵葡萄糖产生的代谢产物有利于改善油藏中的微环境。菌株SC-2与T.mathranii subsp.mathranii 11426T的最适pH和最大耐受NaCl浓度有所不同,且二者的(G+C)mol%含量差异较大。[Objective] We studied physiological, biochemical properties and metabolites of Thermoanaerobacter mathranii SC-2 from oil-field water in Shengli oilfield. [Methods] Strain SC-2 was isolated by Hungate anaerobic technique. Through physiological, biochemical and phylogenetic analysis, the strain was identified. Metabolites were analyzed by gas chromatogram. [Results] The cells were Gram-negative, rod-shaped, spore-forming. Growth was observed in the temperature range from 40 to 75℃ （optimum 70℃） and pH range from 5.5 to 9.5 （optimum 6.5）. The isolate grew in the presence of 0%-5% NaCl with an optimum without NaCl at pH 7.0 and 65℃. Strain SC-2 used many carbohydrates as carbon sources, including glucose and xylose. Metabolites of glucose were ethanol, acetate, propionate, lactate, CO2 and H2. Based on 16S rDNA studies, strain SC-2 was most close to T. mathranii subsp, mathranii11246^T with 99.85% similarity. More ethanol and acetate were produced at initial pH 8.0 than yields at other pH. Yeast extract could significantly increase ethanol and acetate yields. In addition, ethanol （4%） added in the medium obviously inhibited its growth. Strain.[Conclusion] SC-2 was extremely thermophilic, halotolerant anaerobe.

关 键 词：热厌氧细菌 鉴定 产酸、产醇特性 

分 类 号：Q93[生物学—微生物学]