铜绿假单胞菌多重耐药基因的筛选及鉴定  

Screen and identification of genes involved in multidrug resistance in Pseudomonas aeruginosa

作　　者：赵兴艳[1] 梁海华[1] 沈立新[1] 董兆麟[1] 段康民[1] 

机构地区：[1]西北大学生命科学学院西部资源及现代生物技术教育部重点实验室,西安710069

出　　处：《微生物学报》2008年第8期1019-1024,共6页Acta Microbiologica Sinica

基　　金：国家自然科学基金(30470098);重大基础研究前期研究专项(2004CCA01700)~~

摘　　要：【目的】研究铜绿假单胞菌中与耐药性相关的基因。【方法】筛选转座突变体文库中对多种抗菌药物敏感的突变体,通过随机PCR、核苷酸测序及序列比对确定突变体中转座子的插入位点及其破坏的基因。【结果】筛选得到2株对多种抗菌药物敏感的突变体,其中被破坏的基因分别为功能未知的新基因PA2580和PA2800。【结论】PA2580和PA2800可能分别通过参与细胞氧化还原作用和细胞壁合成进而与铜绿假单胞菌耐药性相关。[Objective] To identify genes involved in multidrug resistance in Pseudomonas aeruginosa. [Methods] We constructed and screened a transposon mutation library and obtained mutants that exhibited decreased drug resistance. The insertion sites of the transposon were identified by arbitrary PCR and subsequent DNA sequencing. [Results] Two mutants which became more susceptible to several antibacterial agents than the wild type were identified as mutants with transposon insertion in gene PA2580 and PA2800 respectively. The functions of these two genes are described as unknown in P. aeruginosa database. [Conclusion] PA2580 and PA2800 are involved in drug resistance, possibly through their roles in redox reactions and cell envelope biogenesis.

关 键 词：铜绿假单胞菌 转座突变 多重耐药性 

分 类 号：R378[医药卫生—病原生物学]