用生物信息学方法预测猪链球菌2型05ZYH33株的脂蛋白  

Putative lipoproteins of Streptococcus suis type 2 identified by bioinformatic genome analysis

作　　者：孙理云[1] 

机构地区：[1]河南科技大学动物科技学院,洛阳471003

出　　处：《微生物学报》2008年第8期1104-1109,共6页Acta Microbiologica Sinica

基　　金：河南科技大学人才科研基金(05-010);河南科技大学校科学研究基金(2006ZY042)~~

摘　　要：【目的】鉴别已公布基因组序列的SS2 05ZYH33株的脂蛋白(Lpp)。【方法】首先从Genbank获取由SS2 05ZYH33基因组推测的蛋白质氨基酸序列,然后利用Lpp预测软件PrositeScan和DOLOP预测05ZYH33株的Lpp,采用SignalP 3.0HMM、PrediSi、Phobius、LipoP-HMM和TMHMM version2.0分析预测的Lpp的信号肽,然后经"多数票决法"确定Lpp;最后利用InterProScan和BlastP服务器对鉴定的Lpp进行功能分析。【结果】鉴定出34种Lpp,占SS2致病株05ZYH33蛋白质组的1.555%。结构与功能分析结果表明,最大的一类Lpp是ABC运输蛋白的底物结合蛋白,共有16种,其中YP_001197586、YP_001197918、YP_001198449、YP_001199435、YP_001197482、YP_001199452和YP_001198914等7种基因可与其他成分组成完整的ABC运输蛋白操纵子,这些Lpp在SS2获取糖、氨基酸和金属离子等营养物质方面具有重要作用。YP_001197698与无乳链球菌的黏附素Lmb和化脓链球菌的黏附素Lbp及YP_001198710与肺炎链球菌的SlrA高度同源,推测它们与黏附功能有关,为SS2新的毒力因子;其他Lpp包括酶、参与蛋白质折叠过程的Lpp及功能未知的Lpp。【结论】这些资料提示Lpp在SS2的生理和致病性方面具有重要作用。[Objective] To identify putative lipoproteins of Streptococcus suis serotype 2 （SS2）. [Methods] First, I used the ScanProsite feature of Prosite and the website Database Of bacterial LipOProteins（DOLOP） to identify putative lipoproteins in the recently published genome of SS2 strains 05ZYH33, then validated the identified putative lipoproteins to exclude the false positive by a ＇majority vote＇ approach following analysis with standard tools for signal peptide verification Finally, putative functions were attributed to individual lipoproteins by reference to the identification of conserved domains of lnterPro. Homologous proteins were identified by unfiltered BlastP homology searches （including conserved domain detection）. [Results] Among the 38 identified putative lipoproteins, 34 were validated as lipoprotein and 4 as false positive lipoproteins. The largest functional category （16/34, 44%） of lipoproteins was that predicted to comprise of substrate binding proteins of ATP-binding cassette transport systems. Other roles included lipoproteins that participated in adhesion （YP_001197698 and YP_001198710）, protein export and folding. [Conclusion] Lipoproteins contributed to the physiology of SS2 and influenced its virulence.

关 键 词：脂蛋白 猪链球菌2型 生物信息学 毒力因子 

分 类 号：S852.61[农业科学—基础兽医学]