艾灸对D-半乳糖致小鼠衰老模型抗衰老作用的研究  

Effects of Moxibustion on Cerebral Cortical Nitric Oxide Level and Nitric Oxide Synthase Activity in Aging Mice

作　　者：赵利华[1] 文建军[1] 杨柯[2] 李爱媛[2] 

机构地区：[1]广西中医学院第一附属医院针灸科,南宁530023 [2]广西中医学院中药学院,南宁530001

出　　处：《针刺研究》2008年第4期255-257,261,共4页Acupuncture Research

摘　　要：目的:探讨艾灸延缓衰老的作用机制。方法:33只3月龄昆明小鼠随机分为模型组、生理盐水对照组、艾灸组,另选16月龄小鼠作为自然衰老组(实验结束为18月龄)。模型组、艾灸组小鼠连续42d颈背部皮下注射D-半乳糖造成衰老模型,生理盐水对照组小鼠连续42d颈背部皮下注射等量生理盐水。注射12d后艾灸组选取"足三里""悬钟"两穴进行米粒大艾柱灸治疗,每穴每次3炷,隔日1次,共15次。用硝酸还原酶法测定小鼠大、小脑皮层一氧化氮(NO)含量,用双波长分光光度法测定小鼠大、小脑皮层一氧化氮合酶(NOS)活力。结果:D-半乳糖致衰老小鼠和18月龄自然衰老小鼠其脑皮层NO含量和NOS活力都呈增高的趋势,艾灸组小鼠脑皮层NO含量和NOS活力水平较模型组和自然衰老组明显降低(P<0.05,0.01)。结论:艾灸"足三里""悬钟"可以降低D-半乳糖致衰老小鼠脑皮层中NO含量和NOS活力,从而抑制了增高的NO产生的细胞毒性,起到延缓衰老的作用。Objective To explore the anti-aging effect of moxibustion of ＂Zusnali＂ （ST 36） and ＂Xuanzhong＂ （GB 39） in D-galactose induced aging mice. Methods Thirty-three female Kunming mice （3 months old） were randomly divided into model group （n = 11 ） ,saline control group （n = 10）, and moxibustion group （ n = 11 ）. In addition, another 10 female mice （ 16 months old） were used as the physic-senility control （PSC） group. Senescent mouse model was established by subcutaneous injection of D-galactose （120 mg/kg· d^-1） for 42 days. From the 13^th day on after the first injection, moxibustion （3 cones/acupoint） was applied to bilateral ST36 and GB39, once every other day for one month. At the end of the treatment, the mice were killed under anesthesia for collecting cerebral cortex and cerebellar cortex separately. NO content and NOS activity were assayed by using nitrate reductase method and chemico-chromatometry respectively after homogenate and centrifugalization. Results Compared with saline control group, cerebral NO contents and NOS activity in physic-senility group and model group, cerebellar NO content in model group, cerebellar NOS activity in physic-senility and model groups increased considerably （P〈0.05, 〈0.01）. In comparison with model group, cerebral and cerebellar NO contents and NOS activity of moxibustion group lowered significantly （P〈0. 05,0. 01）. Conclusion Moxibustion of ST36 and GB39 can significantly suppress aging induced increase of both NO content and NOS activity in cerebral and cerebellar cortical tissues in aged mice, suggesting an effective anti-aging effect of moxibustion.

关 键 词：艾炷灸 一氧化氮 一氧化氮合酶 大脑皮层 小脑皮层 

分 类 号：R245.81[医药卫生—针灸推拿学]