青蒿琥酯诱导人白血病细胞K562胀亡和凋亡作用的研究  

The oncosis and apoptosis-inducing effect of artesunate on the K562 human leukemia cell line

作　　者：田雪莲[1] 王莎莉[1] 

机构地区：[1]重庆医科大学基础医学院生理学教研室,重庆400016

出　　处：《重庆医科大学学报》2008年第7期796-798,846,共4页Journal of Chongqing Medical University

基　　金：重庆市科委攻关项目[30472253]

摘　　要：目的:探讨青蒿琥酯(Artesunate,ART)对人慢性粒细胞性白血病细胞株K562增殖抑制的影响。方法:采用MTT比色法检测青蒿琥酯对体外培养的K562细胞的生长抑制作用,光、电镜下观察青蒿琥酯作用后的K562细胞形态学变化特征,流式细胞仪检测在不同浓度、不同时间作用下青蒿琥酯诱导K562细胞的细胞胀亡率和凋亡率。结果:青蒿琥酯对K562细胞有明显的增殖抑制作用,24h、48h、72h的半数抑瘤浓度IC5(0μg/ml)分别为65.17、31.63、10.51。青蒿琥酯主要引起K562细胞的胀亡和凋亡变化,但胀亡率与凋亡率无明显差别。结论:青蒿琥酯主要通过胀亡和凋亡2种方式抑制K562细胞增殖,并呈明显量效时效关系。Objective：To investigate the effect of artesunate（ART） on human leukemia cell line K562. Methods：Inhibition of proliferation was measured by a colorimetric MTT assay. The morphological changes of K562 were observed under a light microscope and an electron microscope. Flow cytometry assay detected the ratio of apoptosis and oncosis of K562 cell lines which were induced at three different times by three different concentrations of ART. Results：ART extract clearly inhibited the proliferation of K562. The 50% effective dose evaluated on 24h,48h,and 72h of ordinal exposure to the extract,were 65.17 μg/ml, 31.63μg/ml,and 10.51μ g/ml, respectively, ART extract-treated cells exhibited morphological changes typical of oncosis and apoptosis. Futheremore,the ratio of apoptosis was not different from to that of oncosis. Conclusion：Artesunate can control proliferation of the K562 human leukemia cell line in two forms：oncosis and apoptosis. The ratio of oncosis and apoptosis present typical dose-dependence and time-dependence.

关 键 词：青蒿琥酯 K562 胀亡 凋亡 

分 类 号：R285.5[医药卫生—中药学]