黄芪、赤芍对小鼠免疫性肝损伤的保护作用  

An Experimental Study on the Protective Effect of Membranous milkvetch Root and Red Peony Root on Immunological Liver Injury in Mice

作　　者：陈英利[1] 邢杰[1] 吕跃山[1] 于洋[1] 

机构地区：[1]哈尔滨医科大学大庆校区检验系,黑龙江哈尔滨163319

出　　处：《贵阳医学院学报》2008年第4期357-359,共3页Journal of Guiyang Medical College

基　　金：黑龙江教育厅面上项目(11511193)

摘　　要：目的:探讨黄芪、赤芍注射液对卡介苗(Bacillus Calmette Guerin,BCG)和脂多糖(lipopolysaccharide,LPS)诱导的小鼠免疫性肝损伤模型的保护作用。方法:采用静脉注射BCG和LPS制备小鼠免疫性肝损伤模型,观察黄芪、赤芍注射液单用及两药配伍对小鼠免疫性肝损伤的影响,检测血清谷丙转氨酶(ALT)、谷草转氨酶(AST)、乳酸脱氢酶(LDH)及小鼠肝组织超氧化物歧化酶(SOD)及丙二醛(MDA)的含量。结果:黄芪、赤芍注射液及二者配伍应用均显著降低免疫性肝损伤小鼠血清ALT、AST、LDH含量,明显提高肝组织SOD活性,降低MDA活性,配伍用药的小鼠ALT、AST、LDH、SOD、MDA五项指标变化均优于黄芪注射液单用(P<0.05、P<0.05、P<0.05、P<0.01、P>0.05)、优于赤芍注射液单用(P<0.01、P>0.05、P<0.05、P<0.01、P<0.05)。结论:黄芪、赤芍注射液对BCG和LPS所致免疫性肝损伤有保护性作用,黄芪注射液配伍赤芍注射液对免疫性肝损伤保护性作用优于两药单用。Objective： to study the protective effect of milkvetch root injection, red peony root injection and their combination to mouse liver injury induced by bacillus calmettc-guerin vaccine （BCG） and Lipopolysaccharide （LPS）. Methods： The immunologically hepatic damage models were induced with injection of BCG ＋ LPS. After the injection of milkvetch root only, red peony root only, or their combination, the recovery of acute liver injury were observed by measuring serum levels of ALT, AST, LDH and superoxide dismutase （SOD） activity, and malond iadehyde （MDA）. Results： Milkvetch root injection, red peony root injection, and their combination significantly decreased the levels of serum ALT, AST, LDH and MDA, but enhanced SOD activity in the liver, and the the curative results （indicated as levels of ALT, AST, LDH, SOD, MDA） of combination injection significantly excelled those of both milkvetch root injection （P 〈0. 05, P 〈0. 05, P 〈0. 05, P 〈0. 01, P 〉 0.05）alone or red peony root injection（P 〈0.01, P 〈0. 01, P 〈0. 05, P 〈0. 01, P 〈0.05） alone. Conclusions： Milkvetch root injection and red peony root injection can protect liver from acute injury induced by BCG ＋ LPS in mice. The combined use of milkvetch root injection and red peony root injection can produce significantly beter protective effect than their sigle use respectively.

关 键 词：免疫性肝损伤 黄芪 赤芍 小鼠 

分 类 号：R285.5[医药卫生—中药学] Q575.1[医药卫生—中医学]