闽产巴戟天中促进骨髓基质细胞增殖成分的初步研究  

Preliminary Study on the Proliferating Bone Marrow Mesenchymal Stem Cells Constituents of Radix Morindae Officinalis Produced in Fujian Province

作　　者：凌昆[1] 郭素华[2] 赵诣[2] 

机构地区：[1]福建中医学院,福州350108 [2]福建中医学院药学系,中药学福建省高等学校重点实验室,福州350108

出　　处：《中国现代应用药学》2008年第4期271-272,共2页Chinese Journal of Modern Applied Pharmacy

基　　金：国家自然科学基金资助项目(项目编号:30572321);2007年度陈可冀中西医结合发展基金(第三批)中西医结合基础福建省重点实验室开放课题基金(项目编号:CKJ2007021、ZXYJH200711)

摘　　要：目的初步研究巴戟天中促进骨髓基质细胞增殖的成分。方法采用MTT法检测,从巴戟天中分离的各组分培养大鼠骨髓基质细胞(BMSCs)72 h后的A值。结果巴戟天中的蒽醌苷类成分无论极性大小,对促进BMSCs的增殖都有效,且正丁醇中极性较大的蒽醌苷类成分效果更好一些。结论巴戟天中含有能促进骨髓基质细胞增殖的成分,值得进一步深入研究。OBJECTIVE To study the constituents of radix morindae officinalis that can proliferate bone marrow mesenchymal stem cells, preliminarily. METHODS According to the MTT method, the OD values of rat bone marrow mesenehymal stem cells cultured by different constituents separated fi＇om radix morindae officinalis for 72 hours were detected. RESULTS Anthraquinone glycosides extracted from radix morindae officinalis can proliferate bone marrow mesenchymal stem cells. And anthraquinone glycosides extracted through n-butanol have better effect. CONCLUSION Radix morindae officinalis contain constituents that can proliferate bone marrow mesenchymal stem cells and worth farther research.

关 键 词：巴戟天 骨髓基质细胞 MTT法 初步研究 

分 类 号：R965.1[医药卫生—药理学] R931.7[医药卫生—药学]