HPLC测定葶苈降血脂胶囊中熊果酸的含量  

Determination of Ursolic Acid in Tingli Jiangxuezhi Capsules by HPLC

作　　者：王威[1] 李红岩[1] 张慧丽[1] 王艳艳[1] 

机构地区：[1]吉林省中医中药研究院,长春130021

出　　处：《中国现代应用药学》2008年第4期331-333,共3页Chinese Journal of Modern Applied Pharmacy

摘　　要：目的建立高效液相色谱法测定葶苈降血脂胶囊中熊果酸的含量。方法采用Diamonsil ODS色谱柱；流动相为乙腈-甲醇-水（65：10：25，每100mL加乙酸铵0．5g）；检测波长为220nm。结果熊果酸进样量在1．592～7．960μg内与峰面积积分值呈良好的线性关系（r=0．9999）；平均加样回收率为99．80％（RSD=1．89％，n=6）。结论方法简便、准确，可作为该制剂的质量控制方法。OBJECTIVE To establish an HPLC method for the determination of ursolic acid in Tingli Jiangxuezhi capsules. METHODS The Diamonsil ODS column was used as the stationary phase with the mobile phase of acetonitrile-methanol-water（65：10 ： 25, ammonium acetate 0.5 g · 100 mL^ -1 ）. The detection wavelength was 220 nm. RESULTS The linear range of ursolic acid was 1. 592 - 7. 960 μg with the correlation coefficient 0. 999 9 between the injection quantity and the peak area. The average recovery was 99.80% with the RSD of 1.89% （n = 6）. CONCLUSION The method is convenient and accurate. It can be used for the quality control of ursolic acid in Tingli Jiangxuezhi capsules.

关 键 词：熊果酸 葶苈降血脂胶囊 高效液相色谱法 

分 类 号：R177.7[医药卫生—妇幼卫生保健] R917.101[医药卫生—公共卫生与预防医学]