DGGE技术在瘤胃微生物多态性研究中的应用  

Application of Denaturing Gradient Gel Electrophoresis in Rumen Microbial Polymorphism

作　　者：李启琳[1] 李燕鹏[1] 王士长[1] 

机构地区：[1]广西大学动物科学技术学院,广西南宁530004

出　　处：《家畜生态学报》2008年第3期101-103,共3页Journal of Domestic Animal Ecology

基　　金：广西自然科学基金资助项目(桂科攻0428005-19A)

摘　　要：利用传统的方法很难鉴别瘤胃微生物的差异,变性梯度凝胶电泳(DGGE)技术就很好地解决了该问题。DGGE是一种新型的用于检测核酸变异和点突变的电泳方法,具有可靠性强、重现性高、方便快捷等优点,可应用于瘤胃微生物群落多样性和动态性分析。The difference between the microbial community inhabiting in the rumen which can not be i-dentified by traditional methods, but denaturing gradient gel electrophoresis （DGGE） made it possible to i-dentify. The DGGE is a new and powerful electrophoresis method for analysis of sequence variations and separation of nucleic acids. The technique is reliable, reproducible, rapid and inexpensive. It is well estab-lished molecular tools in microbial molecular ecology that allows the study of diversity and dynamics of microbial communities.

关 键 词：变性梯度凝胶电泳 瘤胃微生物 多态性 

分 类 号：S811.5[农业科学—畜牧学]