低剂量辐射对人骨髓间充质干细胞生物学特性的影响  

Effect of low dose radiation on biological characteristics of human mesenchymal stem cells from bone marrow

作　　者：杨岩[1] 陈晓[1] 李薇[1] 王冠军[1] 

机构地区：[1]吉林大学第一医院血液肿瘤科,吉林长春130021

出　　处：《吉林大学学报（医学版）》2008年第1期28-31,共4页Journal of Jilin University：Medicine Edition

基　　金：国家自然科学基金资助课题(30370439);吉林省长春市科技计划项目资助课题(04-07SF081)

摘　　要：目的:探讨低剂量辐射(LDR)对骨髓间充质干细胞(BM-MSC)生物学特性的影响。方法:应用体外培养传代的P4、P5、BM-MSC,分别采用剂量为50、75和100mGyX射线照射(剂量率为12.5mGy·min-1),检测不同剂量照射后BM-MSC生长、细胞周期与凋亡的变化。结果:与对照组比较,50、75和100mGy照射后第5天BM-MSC生长进度明显加快(P<0.05);与同一时间对照组比较,50、75和100mGy照射BM-MSC后,G0/G1期细胞百分率减少(P<0.05),照射后72h降至最低;S期细胞百分率在照射后48和72h逐渐明显增多(P<0.05),以75mGy照射后72h,S期细胞百分率增多最明显(68.88%);而细胞凋亡的变化是50、75和100mGy照射后24和48h有增多趋势,照射后72h凋亡细胞百分率有减少趋势。结论:LDR对BM-MSC有兴奋性效应。Objective To explore the effect of low dose radiation （LDR） on biological characteristics of human mesenchymal stem cells from bone marrow （BM-MSC）. Methods The P4, P5, BM-MSC were exposed to X rays at the dose of 50, 75, and 100 mGy （dose rate 12. 5 mGy·min^-1）. The cell growth, cell cycle and apoptosis of BM-MSC treated with LDR were determined. Results Compared iwth control group, the cell growth rates of BM- MSC treated with LDR （50, 75, 100 mGy） were obviously increased from the fifth day （P〈0.05）. Compared with control group at the same time, the percentage of G0/G1 stage cells gradully decreased （P〈0.05）, and after treated for 72 h, the growth rate was the lowest. However, the percentage of S stage cells e gradually increased after treated for 48 h and 72 h （P%0. 05） . The most one was 75mGy group at 72 h （68.88G）. The percentages of apoptotic cells had increasing tendency at 24 h and 48 h in all dose groups, while had decreasing tendency at 72 h. Conclusion LDR has hormesis effect on BM-MSC.

关 键 词：骨髓间充质干细胞 低剂量辐射 细胞周期 凋亡 

分 类 号：R817.1[医药卫生—影像医学与核医学]