3,4,5-三羟基苯甲酸对肿瘤细胞凋亡及细胞周期进程的影响  

Effects of purified product from trapa manshurica fler on apoptosis and cell cycle progression of tumor cells in vitro

作　　者：吕喆[1] 龚守良[1] 牛凤兰[2] 王学东 

机构地区：[1]吉林大学公共卫生学院卫生部放射生物学重点实验室,吉林长春130021 [2]吉林大学公共卫生学院卫生化学教研室,吉林长春130021 [3]吉林省长春市朝阳区疾病控制中心,吉林长春130021

出　　处：《吉林大学学报（医学版）》2008年第1期90-92,共3页Journal of Jilin University：Medicine Edition

基　　金：吉林省科技厅重点项目资助课题(20070424)

摘　　要：目的:观察不同剂量3,4,5-三羟基苯甲酸(TAD)对体外人肝癌SMMC-7721细胞凋亡及细胞周期进程的影响,探讨其对肿瘤生长抑制的可能机制。方法:实验分0、3.125、6.250、12.500和25.000μgTAD处理组,应用透射电镜观察肿瘤细胞凋亡,流式细胞术检测TAD处理后肿瘤细胞凋亡及细胞周期进程的变化。结果:电镜证实,25.000μgTAD组肿瘤细胞呈典型凋亡特征,即核呈碎裂状,线粒体嵴断裂,胞核及细胞质呈空泡化改变;流式细胞仪检测,不同剂量TAD分别处理细胞后其凋亡百分数均高于对照组(P<0.01);与对照组比较,随TAD剂量的增加G0/G1期细胞百分数增加,其中25.000μgTAD组发生明显G1阻滞(P<0.01);S期细胞百分数逐渐降低,其中25.000μgTAD组明显低于对照组(P<0.05)。结论:TAD可使肝癌SMMC-7721细胞凋亡和G1期阻滞,其对肿瘤生长的抑制作用可能通过G1期阻滞引起细胞凋亡实现的。Objective To observe the effects of 3, 4, 5-trihydroxybenzoic acid dimmer （TAD） in different doses on apoptosis and cell cycle progression of liver cancer SMMC-7721 cells in vitro and explore its possible mechanism of inhibiting tumor growth. Methods The experiment was divided into 0, 3. 125, 6. 250, 12. 500 and 25. 000 μg TAD groups. Apoptosis was observed with JEM-1200EX transmission electron microscope （TEM）. The changes of apoptosis and cell cycle progression were measured with flow cytometry. Results The typical features of apoptosis were found in tumor cells, the nuclei were broken to pieces, mitochondrion cristae were disrupted and vacuoles were showed in nucleus and cytoplasm in 25. 000 μg TAD group observed under TEM. Meantime, the apoptotic percentages of the cells treated with 3. 125--25. 000 /,g TAD were increased significantly as compared with control group （P〈0. 001）. And the percentages of G0/G1 phase cells treated with TAD were increased, it was significantly higher in 25. 000 μg TAD group than that in control group （P〈0. 001） ; while the percentages of S phase cells treated with TAD were decreased, especially in 25. 000 μg TAD group as compared with control group （P〈 0.05）. Conclusion TAD could induce liver cancer SMMC-7721 cell apoptosis and G1 phase arrest. The inhibitory effect of TAD on tumor growth could be due to apoptosis caused by G1 phase arrest.

关 键 词：三羟基苯甲酸 细胞凋亡 细胞周期 

分 类 号：R329.28[医药卫生—人体解剖和组织胚胎学]