水稻条纹叶枯病毒RNA干扰载体的构建  

Construction of Expression Vector for Anti Stripe Virus of Rice by RNA Interference

作　　者：张恭[1] 刘立峰[1] 周维[2] 王海光[2] 马峙英[1] 

机构地区：[1]河北农业大学农学院,河北保定071001 [2]中国农业大学,农业部作物基因组学与遗传改良重点实验室,北京100094

出　　处：《华北农学报》2008年第4期10-13,共4页Acta Agriculturae Boreali-Sinica

基　　金：河北省自然科学基金资助项目(C2006000447)

摘　　要：利用已公布的水稻条纹叶枯病毒的序列和siRNA Target Finder软件,获得了168条siRNA片段,利用这些片段在水稻基因组中BLAST(Basic local alignment search tool),最终获得6条与水稻基因组完全不匹配的干扰序列。选择其中的2条,通过化学合成干扰序列,利用pCAMBIA1301质粒构建了RNA干扰载体pASV1301-1,pASV1301-2,并成功地转化EHA105农杆菌,为进一步转化水稻、探索利用RNA干扰技术防治水稻条纹叶枯病奠定了基础。By using the sequence of stripe virus published on line and the software of siRNA Target Finder, we obtained 168 siRNA segments. After BLAST Scan of rice genome, 6 segments were selected and 2 were chemically synthesized and used to construct of expression vector （pASV1301-1, pASV 1301-2）intergrated with pCAMBIA1301 plasmid. The recombined plasmid was successfully transformed into agrobacterium of EHA105, which lay a foundation for the transformation of rice and the prevention of stripe virus disease of rice by RNA interference.

关 键 词：水稻 RNA干扰 载体构建 抗病毒 

分 类 号：S432.41[农业科学—植物病理学]