新城疫病毒河北分离株F基因的克隆及序列分析  

Cloning and Sequence Analysis of F Gene of Newcastle Disease Virus Isolated from Hebei Province

作　　者：张丽青[1] 王爱华[2] 张秀珊[1] 潘丽娜 杜冬华 周静[2] 闫书彩[1] 孙继国[1] 

机构地区：[1]河北农业大学动物科技学院 [2]河北北方学院动物科技学院医学系,河北张家口075000 [3]承德市双桥区农业畜牧局,河北承德067000

出　　处：《华北农学报》2008年第4期19-22,共4页Acta Agriculturae Boreali-Sinica

摘　　要：参照GenBank中NDV的核酸序列设计一对引物，利用RT-PCR扩增F基因并得到长约1．7kb的全基因片段，并将其构建到pMD-19T载体上。核苷酸序列测定结果表明：该基因片段全长1662bp，编码553个氨基酸，与GenBank下载的13株参考毒株F基因比较，核苷酸和氨基酸序列同源性分别为97．2％。99．2％和96．9％。98．7％；但与LaSota、F48E9及B1等常见疫苗株的氨基酸同源性仅为88．6％～91．5％。NDV分离株F蛋白裂解位点的氨基酸序苑为112R．R-Q—K—R—F—I—G119，属于基因ⅦNDV。One pair of primers for amplifying the F gene of NDV on GenBank were designed, with which the predicted 1.7 kb fragment had been amplified via RT-PCR from NDV strain Hebei.The 1.7 kb fragment was inserted into pMD19T vector. DNA sequence analysis showed that the cloned fragment consisted of 1 662 bp and coding for 553 amino acid. Compaired with 13 strains of NDV on GenBank, neucleitide and amino acid sequence homologies were found to be 97.2% - 99.2 % and 96.9 % - 98.7 % respectively, but the amino acid sequence homologies to other NDV strains such as La- Sota,F48E9 and B1 were only 88.6% -91.5% .The deduced amino acid sequence of the cleavage site of F protein of the NDV isolates were 112R-R-Q-K-R-F-I-G119. the NDV isolates belonged to the genetype Ⅶ.

关 键 词：新城疫病 F基因 克隆 序列分析 

分 类 号：S432.41[农业科学—植物病理学]