荧光增白剂和增效蛋白对粘虫核型多角体病毒的增效作用  

Enhancing Effects of Fluorescent Brightener 28 and Enhancin on Mythimna separata Nucleopolyhedrovirus

作　　者：陈涛[1,2] 王俊平[2] 李淑文[2] 周洪旭[2] 李国勋[1,2] 

机构地区：[1]河北农业大学,河北省农业病虫害生防工程技术中心,河北保定071001 [2]青岛农业大学害虫生物防治研究所,山东青岛266109

出　　处：《华北农学报》2008年第4期23-27,共5页Acta Agriculturae Boreali-Sinica

基　　金：国家重点基础研究发展计划“973”项目(2003CB114201);山东省自然科学基金资助项目(Y2007D69)

摘　　要：离体和活体处理粘虫幼虫围食膜后,通过SDS-PAGE分析其围食膜蛋白条带的变化、观察其形态学特征、生物测定等方法研究了荧光增白剂FB28和棉铃虫颗粒体病毒增效蛋白(En-Ha)对粘虫幼虫围食膜的破坏作用及其对粘虫核型多角体病毒(MsNPV)的增效作用。结果表明,FB28离体处理围食膜能够解离大部分围食膜蛋白,幼虫摄取含有1%FB28的饲料4 h后,围食膜破裂,呈现出蓝色的荧光,高分子量蛋白条带消失。En-Ha离体处理围食膜,能够降解高分子量蛋白,出现高分子量蛋白条带消失,低分子量蛋白条带出现的现象;幼虫摄取含有9μg/mL En-Ha饲料7 h后,围食膜极易断裂,高分子量蛋白条带消失。经过一段时间恢复实验后,FB28和En-Ha处理的幼虫围食膜都能够恢复正常,其破坏作用是短暂的。生物测定结果表明,FB28和En-Ha均能够通过破坏围食膜完整性提高MsNPV的感染率,使LC50显著降低。The disruptive effects of Fluorescent Brightener 28 （FB28） and Heliothis armigera granulovirus enhancing （En-Ha） on the peritrophic membrane（PM） of larvae and enhancing effects on MsNPV in Mythima separata were studied through SDS-PAGE, structural observation and bioassay. The results indicated that proteins could be dissociated from the PM in vitro by FB28. After the larvae were reared with diets containing 1% FB28 for 4 h, bluish fluorescence appeared on the disrupted PM. SDS-PAGE of PM treated with FB28 in vivo showed that some proteins with high molecular weight disappeared. After the PM was treated with En-Ha in vitro, some proteins with high molecular weight disappeared and some with lower molecular weight appeared, which indicated En-Ha could degrade the high-molecular-weight proteins from the PM.The PM became fragile after the larvae were reared with diets containing 9μg/mL En-Ha for 7 h,and SDS-PAGE showed that some proteins with high molecular weight disappeared similar to the profile treated by FB28. However, the experiments testified that the disruptive effect could be restored after rearing the larvae on the diet without FB28 and EnHa. The bioassay showed that FB28 and En-Ha could enhance the vitality of MsNPV and significantly decreased LC50 by degrading the integrity of PM.

关 键 词：荧光增白剂 增效蛋白 粘虫核型多角体病毒 围食膜 增效作用 

分 类 号：S432.41[农业科学—植物病理学]