小麦与叶锈菌互作前后基因组甲基化模式分析  

Analysis of DNA Methylation Related on the Interaction between Wheat and Wheat Leaf Rust

作　　者：付胜杰[1] 王晖[2] 冯丽娜[1] 李星[1] 王坤[1] 杨文香[1] 刘大群[1] 

机构地区：[1]河北农业大学植物保护学院植物病理系,河北省农作物病虫害生物防治工程技术研究中心,河北保定071001 [2]济宁医学院,山东济宁272013

出　　处：《华北农学报》2008年第4期28-31,共4页Acta Agriculturae Boreali-Sinica

基　　金：国家“973”专项(2005CCA01600);国家“十一五”支撑计划(2006BAD08A05)

摘　　要：首次使用甲基化敏感扩增多态性(MSAP)技术分析两种小麦材料近等基因系TcLr19及其感病亲本Thatcher的甲基化水平,同时比较了苗期接种叶锈菌生理品种THTT前后基因组DNA胞嘧啶甲基化模式。60对选扩引物对接种前后的小麦DNA进行全基因组筛选,共得到3 554个片段。其中998个片段是两种甲基化模式中的一种,小麦近等基因系TcLr19及其感病亲本Thatcher的甲基化水平约为28.1%。在所有的引物中,并没有直接分离得到接菌前后的甲基化模式的差异。结果初步表明,叶锈菌可能并没有诱导植物基因组DNA胞嘧啶位点的甲基化模式变化。DNA methylation is known to play an important role in the regulation of gene expression in eukaryotes. The wheat near-isogenic line TcLr19 and Thatcher is race-specific resistance and compatible in the interaction with Paccinia triticina respectively. Using methylation-sensitive amplified polymorphism（MSAP） analysis, we compared the patterns of cytosine methylation in TcLr19 and Thatcher that had been inoculated with Puccinia triticina, subjected to left untreated. In all, 3 554 DNA fragments, each representing a recognition site cleaved by either or both of two isoschizomers, were amplified using 60 pairs of selective primers. A total of 998 sites were found to be one of the methylated.The results suggest that no differences in cytosine methylation exist between seedlings inoculated and those unchallenged.

关 键 词：DNA甲基化 小麦叶锈菌 甲基化敏感扩增多态性 抗病性 

分 类 号：S512.01[农业科学—作物学]