鞘翅目害虫-华北大黑鳃金龟围食膜结构的初步研究  

Preliminary Study on Structure of Peritrophic Membrane(PM) from Holotrichia oblita(Coleoptera:Melolonthidae)

作　　者：李帅[1] 李淑文[1] 李长友[1] 周洪旭[1] 李国勋[1] 

机构地区：[1]青岛农业大学害虫生物防治研究所,山东青岛266109

出　　处：《华北农学报》2008年第4期55-59,共5页Acta Agriculturae Boreali-Sinica

基　　金：国家重点基础研究发展计划"973"项目(2003CB114201);山东省自然科学基金资助项目(Y2007D69)

摘　　要：应用光学显微镜和生化技术，研究了华北大黑鳃金龟（Holotrichia oblita）围食膜的基本结构和表面形态，初步分析了围食膜蛋白质的种类，蛋白质和糖的含量，测定了棉铃虫颗粒体病毒增效蛋白对围食膜结构的影响。结果表明，华北大黑鳃金龟围食膜表面平滑致密，富有弹性，但与多数昆虫围食膜相比韧性较差，主要由蛋白质、糖和几丁质组成，其中蛋白质的含量约为36．25％，糖的含量约为8．91％，其他为几丁质等；经SDS—PAGE测定，围食膜的蛋白质种类较多，有28条多肽比较清晰，分子量多在17．6—320．3kDa。棉铃虫颗粒体病毒增效蛋白不能降解华北大黑鳃金龟围食膜中的蛋白质。As an initial step to study the structure of PM from Holotrichia oblita, the basic structure of the PM was analyzed by microscopy and the PM morphological aspects were observed by biochemical technology. The results showed that major components of the PM were proteins accounting for 36.25 %, and carbohydrates accounting for 8.91% by weight besides chintin. PM surface appeared to be smooth, close and highly elastic. Apparently 28 polypetides were separated by SDS-PAGE analysis with molecular weight from 17.6 to 320.3 kDa. Heliothis armigera granulosis virus enhancin could not degraded any proteins by assay in vitro.

关 键 词：华北大黑鳃金龟 围食膜 结构 增效蛋白 

分 类 号：S433.5[农业科学—农业昆虫与害虫防治]