小麦TaNCED1基因植物表达载体的构建  

Construction of Expression Vector of the Triticum aestivum L.TaNCED1 Gene

作　　者：西廷业[1] 王洪刚[1] 后猛[1] 刘海燕[1] 刘树兵[1] 

机构地区：[1]山东农业大学农学院,国家小麦改良中心泰安分中心,作物生物学国家重点实验室,山东泰安271018

出　　处：《华北农学报》2008年第4期77-80,共4页Acta Agriculturae Boreali-Sinica

基　　金：山东省优秀中青年科学家基金科研奖励基金

摘　　要：以植物双元表达载体pCAMBIA2300-35为基础,设计带有酶切位点KpnI和XbaI的一对引物,从克隆载体pGEM-NCED1扩增到目的基因TaNCED1,酶切回收后与同样双酶切的表达载体pCAMBIA2300-35连接,获得表达载体pTaNCED1,并将所构建的载体导入根癌农杆菌GV3101菌株,为该基因的功能鉴定及通过基因工程的方法提高小麦的抗逆性奠定了基础。In this study, Triticum aestivum L.9-Cis-Epoxycarotenoid Dioxygenase gene（ TaNCED1 ） was amplified using specific primers containing restriction enzyme site of KpnI and XbaI. PCR products and the binary expression vector pCAMBIA2300 - 35S were digested by the corresponding restricted enzymes respectively, and linked directionally. The resuiting construction with TaNCED1 was named pTaNCED1. The pTaNCED1 was further introduced into Agrobacterium tumefaciens strain GV3101 for the following-up research such as gene functional identification and gene transformation for wheat stress tolerance improvement.

关 键 词：小麦 9-顺式-环氧类胡萝卜素双加氧酶 脱落酸 

分 类 号：Q786[生物学—分子生物学]