卵泡颗粒细胞维持山羊卵母细胞减数分裂抑制状态的研究  

Maintenance of Meiotic Arrest in Goat Oocyte by Follicular Cells

作　　者：刘利杰[1] 权富生[1] 刘军[1] 张东[1] 刘根胜[1] 张涌[1] 

机构地区：[1]西北农林科技大学生物工程研究所,陕西杨凌712100

出　　处：《华北农学报》2008年第4期119-123,共5页Acta Agriculturae Boreali-Sinica

基　　金：国有高技术研究与发展计划"863"项目资助(2001AA213081)

摘　　要：通过在卵母细胞体外成熟过程中添加不同浓度的卵丘细胞或其外围壁层颗粒细胞进行共培养,对山羊卵母细胞成熟过程中卵泡颗粒细胞对卵母细胞减数分裂抑制作用及作用机制进行了探索。结果表明,成熟液中添加105和106个/mL卵丘细胞或外围壁层颗粒细胞的卵母细胞体外成熟率与对照组相比差异不显著(p>0.05),但添加5×106个/mL(41.2%)和107个/mL(24.6%)卵丘细胞或外围壁层颗粒细胞组的成熟率显著降低(p<0.05);添加5×106和107个/mL卵丘细胞组和颗粒细胞组卵丘不能正常扩展,卵母细胞核状态观察,停留在GV期;Rp-cAMP(Rp-cyclicadenosine monophosphothioate)膜渗透性cAMP对抗剂,诱导了107个/mL的卵丘细胞组(71.6%)和外围壁层颗粒细胞组(71.3%)的卵母细胞体外成熟率与对照组(72.0%)差异不显著(p>0.05)。在山羊卵母细胞体外成熟过程中,添加一定浓度的卵丘细胞及其外层的颗粒细胞可以提高卵母细胞胞质内的cAMP的水平,抑制卵母细胞减数分裂的启动,使卵母细胞停留在GV期。In this experiment, during the process of goat oocyte IVM, by adding different concentration cumulus cell and its surrounding granular cell, we studied the inhibitive effects and its mechanism of follicular cells on meiosis of oocytes. The IVM rate of oocyte was not being affected considerably after adding 105 and 106 cell/mL cumulus cells or surrounding granular cells, contrast to control group（p 〉 0.05）, but were significantly higher than that of 5 × 10^6 cell/mL （41.2%）, 107 cell/mL（24.6% ）cumulus cells and 5 × 10^6 cell/mL（38.5 % ）, 107 cell/mL（23.8% ）surrounding granular cells（ p 〈 0. 05）. In adding 5 × 10^6, 10^7 cell/mL cumulus cells group and surrounding granular cells group, the cumulus could not expand. Nuclear Status Examination, the meiosis of oocytes was maintained in GV stage. Rp-cyclic adenosine monophosphothioate,a membrane-permeable antagonist to cAMP, induced the oocyte IVM rate of 10^7 cell/mL cumulus cells group（71.6 % ）and surrounding granular cells group （71.3 % ）which there was no significant difference among the control group（p 〉 0.05）.during the process of oocyte IVM,adding some concentration cumulus cells and surrounding granular cells would maintain meiotic arrest by increasing the cAMP level of oocyte, make oocyte stay in the GV stage.

关 键 词：山羊 卵泡颗粒细胞 卵母细胞 减数分裂 抑制 

分 类 号：S826[农业科学—畜牧学]