HPLC-ELSD法测定扶芳藤中卫矛醇的含量  

Determination of Dulcitol in Fortune Euonymus Stem by HPLC-ELSD

作　　者：覃洁萍[1] 姚蓉[1] 李芸[2] 王丽丽[1] 许晨霞[1] 李伟[1] 

机构地区：[1]广西中医学院,南宁市明秀东路179号530001 [2]广西中医学院制药厂,530023

出　　处：《广西中医药》2008年第4期58-60,共3页Guangxi Journal of Traditional Chinese Medicine

基　　金：广西自然科学基金资助项目(桂科自0447076)

摘　　要：目的:建立扶芳藤药材中卫矛醇的含量测定方法。方法:采用HPLC法,色谱柱为Lichrospher5-NH2柱(250mm×4.6mm,5μm);流动相为乙腈-水(90:10);流速为1.0ml/min;柱温为28℃;检测器为PL-ELS2100型蒸发光散射检测器(漂移管温度65℃,雾化室温度50℃,气体流量为:0.9L/min)。结果:卫矛醇在2.91～29.1μg范围内,其峰面积的自然对数(Y)与进样量的自然对数(X)呈良好的线性关系,r=0.9998;平均加样回收率100.78%,RSD为2.36%(n=6)。结论:该方法简便,重复性好,结果准确,可作为扶芳藤药材中卫矛醇的含量测定方法。Objective：To establish a method for the determination of dulcitol in Fortune euonymus stem. Method： The way of HPLC-ELSD was used. Chromatographic column was Lichrospher5-NH2 column （250 mm× 4.6 mm, 5μm） and the mobile phase was a mixture of acetonitrile-water （90：10） with 1.0ml.min^-1 as the flow rate. Column temperature was at 28℃ and the detector was PL-ELS2100 ELSD （diift tube Temp：65℃,atomizer chamber Temp：50℃ ,gas flow：0.9 mL/min）. Results： When dulcitol was in the rang of 2.91-29.1μg, the natural logarithm（Y） of peak area and the natural logarithm（X） of sample size showed a satisfactory linear relationship R = 0.9998;the average recovery of application of sample was 100.75% and RSD was 2.3%（n= 6）. Conclusion： The method is easy which has a good repeatability and an accurate result. It can be the method for the determination of dulcitol in Fortune euonymus stem.

关 键 词：卫矛醇 扶芳藤 HPLC 蒸发光散射检测器 

分 类 号：R284.1[医药卫生—中药学]