江苏水稻品种抗稻瘟病遗传多样性研究  

Genetic diversity of rice varieties resistant to rice blast

作　　者：陈志石[1] 陈夕军[2] 熊如意[1] 林玲[1] 徐敬友[2] 程兆榜[1] 

机构地区：[1]江苏省农业科学院植物保护研究所,江苏南京210014 [2]扬州大学农学院,江苏扬州225009

出　　处：《南京农业大学学报》2008年第3期77-80,共4页Journal of Nanjing Agricultural University

基　　金：国家支撑计划项目(2006BAD02A16、2006BAD08A04);江苏省高技术项目(BG2001306)

摘　　要：根据已知的不同抗病基因保守区域设计引物,通过在水稻基因组DNA中扩增和分离有相似序列的DNA片段,可以快速鉴定出候选抗病基因。选择3对扩增条带多样性较高的引物XLRRfor/XLRRrev、S1/AS3和Ptokin1/Ptokin2,对江苏27个水稻品种进行抗病基因同源序列PCR分析(resistance gene analog-PCR,RGA-PCR)。结果显示,3对引物共扩增出清晰可辨的谱带127条,其中多态性带101条,占总数的79.53%。根据聚类分析,3对引物对或引物组合扩增的RGA图谱可将品种分为多种类型,这些RGA图谱类型与稻瘟病菌7个生理小种接种鉴定的抗病表型没有完全的对应关系。To design primers according to conserved domains in those published resistant genes, DNA sequences can be amplified from DNA genome in rice to obtain candidate resistant gene rapidly. 3 primer pairs including XLRRfor/XLRRrev, Ptokinl/Ptokin2 and S1/AS3 were selected to amplify 27 rice varieties from Jiangsu Province. 127 bands were amplified from 27 rice varieties by 3 primer pairs, in which 101 bands were polymorphic, with the polymorphic band frequency of 79.53%. Resistance gene analog- PCR （RGA-PCR） maps of rice varieties amplified by 3 primer pairs or primer pair combinations could be grouped by cluster analysis. There were no completely corresponding relations between these RGA-PCR map groups and resistance phenotypes of rice cultivars.

关 键 词：水稻 抗病基因同源序列(RGA) 抗病表型 遗传多样性 

分 类 号：S511[农业科学—作物学] S435.111.41