慢性饮酒对小鼠卵巢功能及其对eCG反应性的影响  

Effects of chronic ethanol intake on ovarian functions and its response to eCG in the mouse

作　　者：王正朝[1] 钟灵秀[1] 郭宁[1] 张磊[1] 石放雄[1] 

机构地区：[1]南京农业大学动物科技学院,江苏南京210095

出　　处：《南京农业大学学报》2008年第3期155-158,共4页Journal of Nanjing Agricultural University

基　　金：国家自然科学基金项目(30571335);江苏省研究生创新工程项目;南京农业大学SRT项目

摘　　要：为研究酒精对哺乳动物卵巢的生殖毒性作用,利用未成熟雌性小鼠进行了酒精的生殖毒性研究。试验选用24只21日龄断奶未成熟小鼠,随机均分为3组,试验组Ⅰ自由饮用啤酒,试验组Ⅱ饮用稀释的白酒(含体积分数为5%的乙醇),对照组饮用自来水。试验期间检查阴道开张日龄;试验5周后每组分别取4只小鼠用乙醚麻醉后测定体重、卵巢、子宫等指标;同时观察卵巢和肝脏的组织学形态;剩余12只小鼠用孕马血清促性腺激素(eCG)处理,48 h后进行麻醉、称重和组织学观察。结果表明:试验组Ⅰ和Ⅱ小鼠阴道开张日龄显著推迟;eCG处理前试验组Ⅰ和Ⅱ小鼠卵巢和子宫质量与对照组相比显著增加;eCG处理后试验组Ⅰ卵巢质量与对照组相似,但试验组Ⅱ卵巢质量显著低于对照组和试验组Ⅰ,子宫与卵巢呈相似的变化;在肝脏组织形态学上也体现出了酒精的毒性作用。由此可见,慢性饮酒抑制了小鼠卵巢功能,并且对eCG反应性显著降低,表明慢性饮酒对卵巢和肝脏均有直接的毒害作用。Immature female mice were used to study effects of chronic ethanol intake on the mammalian development and its reproductive toxicity. Twenty-four weaned female mice of 21 clay-old were randomly allotted into three groups of eight each. Group Ⅰ were fed with beer, and Group Ⅱ with diluent alcohol （5%） ; while the third group with fresh tap water were used as the control. Four mice from each group were anatomized after anaesthesia with aether, and weights of body, ovary, uterus of each animal were measured. In addition, ovary and liver histology were examined after 5 weeks of treatments. Furthermore, 12 mice left were treated with equine chorionic gnnadotropin （eCG） and then anatomized, weighed and examined histologically 48 h later. The results indicated that the day of vaginal opening was delayed significantly in Group Ⅰ and Ⅱ. The weights of ovary and uterus in Group Ⅰ and Ⅱ were heavier than those in Control before eCG treatment. After eCG treatment, ovarian weights in Control and Group Ⅰ significantly increased, while, ovarian weights in Group Ⅱ kept unchanged and were significantly lighter than that in Control and Group Ⅰ . The observation of liver histology indicated also pathological changes in Group Ⅰ and Ⅱ. Together, chronic ethanol intake represses ovarian functions in the mice, and depresses the response of eCG, implying the directly toxic effects of chronic ethanol on the ovary and liver.

关 键 词：酒精 小鼠 生殖毒性 卵巢 子宫 肝脏 孕马血清促性腺激素(eCG) 

分 类 号：Q492.5[生物学—生理学]