慢性阻塞性肺疾病患者血清Fas/Apo-1及TNF-α与细胞凋亡的相关性研究  

A study on changes of Fas/Apo-1 and TNF-αin the serum of patients suffering from COPD and its clinical significance with cell apoptosis

作　　者：王晓园[1] 许西琳[1] 关键[1] 刘冬[1] 于颍佳[1] 陈艺慧[1] 

机构地区：[1]新疆石河子大学医学院第一附属医院呼吸内科,832000

出　　处：《临床肺科杂志》2008年第10期1294-1295,共2页Journal of Clinical Pulmonary Medicine

摘　　要：目的探讨慢性阻塞性肺疾病(COPD)患者血清可溶性(Fas)及(TNF-α)的变化及相关性。方法30例急性加重期和稳定期COPD患者、25例健康体检者,分别查血常规、测定肺功能,用ELISA法检测血清sFas及TNF-α浓度。结果发现COPD急性加重期组患者外周血中性粒细胞占白细胞总数百分比Neu/Leu%、sFas、TNF-α明显高于COPD稳定期组(P<0.01,P<0.05,P<0.01);COPD急性加重期组患者血清sFas、TNF-α明显高于健康对照组(P<0.05,P<0.01),且在急性加重期组中TNF-α与Neu/Leu%呈正相关(r=0.586,P<0.05)。结论COPD急性加重期及稳定期sFas和TNF-α水平显著升高,提示凋亡参与了COPD的发病,可能是引起肺内炎症细胞浸润及肺实质破坏的主要原因之一。Objective . To investigate the clinical significance of changes of serum levels sFas and TNF-α in patients with chronic obstructive pulmonary disease （COPD）. Methods 30 patients with COPD during acute exacerbation and stable period, and 25 normal persons （ control group） were chosen. Testing the pulmonary function and the ratio of eutrophils/leukocytes Neu/Leu% , enzyme linked immunosorbent assay （ELISA） was used to measure sFas and TNF-α in all. Results The levels of Neu/Leu%, sFas and TNF-α in the COPD group during acute exacerbationit was significantly higher than that in the stable period （P 〈 0.01,P 〈 0. 05,P 〈 0. 01 ）. The levels of sFas, TNF-α in the COPD group during the acute exacerbationit period was higher than that in the healthy controls （ P 〈0.05, P 〈 0. 01 ）. The levels of Neu / Leu% in the COPD group during the acute exacerbationit presented positive correlation notably to TNF-α, （ P 〈 0. 05）. Conclusion The serum sFas and TNF-α levels rise in patients with COPD during the acute exacerbation and stable periods, which indicates that cell apoptosis may play a rule in the course of inflammatory reaction of air passage of COPD.

关 键 词：慢性阻塞性肺疾病 Fas/Apo—1 TNF—α 细胞凋亡 

分 类 号：R563.9[医药卫生—呼吸系统]