芒果苷对心肌缺血再灌注损伤TNF-a、MPO的影响  

Effects of mangiferin of TNF-a and MPO in rats with myocardial ischemia reperfusion injury WANG

作　　者：王超[1] 王国贤[1] 

机构地区：[1]辽宁医学院药理学教研室,辽宁锦州121001

出　　处：《中国医药技术经济与管理》2008年第8期60-65,共6页China Pharmaceutical Technology Economics & Management

摘　　要：目的观察芒果苷对大鼠心肌缺血再灌注损伤(MI/R)TNF-a、MPO的影响。方法50只SD大鼠随机分为5组即假手术组、缺血再灌注模型组、芒果苷10 mg/kg、20 mg/kg、40 mg/kg剂量组。假手术组给予等容量的生理盐水腹腔注射,完成模型全部操作只穿线不结扎。芒果苷10、20、40mg/kg剂量组分别给予相应剂量芒果苷腹腔注射21天,末次给药后一小时用于实验。采用左冠状动脉前降支结扎40 min,再灌120 min建立在体大鼠MIR模型。放免法检测心肌组织肿瘤坏死因子(TNF-a)含量;检测心肌组织髓过氧化酶(MPO)观察中性粒细胞(PMN)浸润。苏木素-伊红(HE)染色观察心肌组织病理改变,透射电镜观察心肌超微结构的改变。结果与模型组比较,芒果苷各剂量TNF-a和MPO含量显著降低(P<0.01或P<0.05)。结论芒果苷对MI/R有保护作用。芒果苷可减轻心肌组织中性粒细胞浸润、抑制炎症因子释放。Objective To investigate the effect of mangiferin on attenuating TNF-α and MPO In rats with myocardial ischemia reperfusion （MIR）injury Methods Fifiy SD rats were randomly divided into five groups sham, model, mangiferin groups of 10 mg/ kg, 20 mg/kg, 40 mg/kg, sham group with equal volume of saline intraperitoneal injected while was given all the procedures except ligation, mangiferin groups were injected with corresponding concentrations of mangiferin intraperitoneal injected for 21 days. one hour after the last administration For the expe -riment. Myocardial ischemia reperfiuson models were obtained by ligated left anterior descendi -ng coronary artery 40 minutes and followed by 120 minutes reperfusion. Detected Myocardial Tumor necrosis factor-a （TNF-a） with P, IA; Detected Infiltration of polymorpho nuclear neutrophi -Is （PMN） in myocardium with Myeloperoxidase （MPO）. observe pathologic changes of myocardium with HE stainning, observed ultrastructure of myocardinm with TEM. Results Compared with the model group, mangiferin group TNF-a , MPOwere significantly lower （P〈0.01, P〈0.05）. mangiferin group could improve myocardial pathologic and ultrastructure and pathologic. Conclusion mangiferin can protect MIR, The myocardial protective mechanism of may be realized by Alleviate Infiltration of polym -errphonuclear neutrophils （PMN） in myocardium ; inhibit inflammatory factor liberation relate.

关 键 词：芒果苷 缺血再灌注损伤 肿瘤坏死因子 心肌髓过氧化物 

分 类 号：R542.2[医药卫生—心血管疾病] R285.5[医药卫生—内科学]