丙型肝炎病毒肝外组织感染与肝外肿瘤的关系  

Study on the relationships between the infection of HCV in extra-hepatic tissues and extrahepatic tumors of chronic hepatitis C patients

作　　者：丁红方[1] 马科[1] 田德英[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院感染科,湖北武汉430030

出　　处：《中西医结合肝病杂志》2008年第4期223-224,228,共3页Chinese Journal of Integrated Traditional and Western Medicine on Liver Diseases

摘　　要：目的:研究丙型肝炎病毒(HCV)在肝外组织感染及复制状况,探讨HCV与肝外肿瘤的关系。方法:采用免疫组化技术对70例慢性丙型肝炎(CHC)患者合并外科疾患的手术切除标本进行HCV抗原标记;RT-PCR检测组织中HCV正、负链RNA。结果:70例CHC合并恶性肿瘤的组织标本37份,HCV抗原阳性率为67.6%(25/37);非恶性肿瘤的组织标本33份,HCV抗原阳性率为24.2%(8/33),两者比较差异有显著性意义(χ2=13.14,P<0.05)。33份HCV抗原阳性的组织中21份(63.6%)检出HCV正链RNA,10份(30.3%)检出HCV负链RNA,且组织中正链和负链HCV RNA的检出与细胞内HCV抗原表达信号呈较好的一致性。结论:HCV可以感染肝外组织,其中部分肝外组织是HCV的复制场所;在CHC患者HCV除诱发原发性肝癌外,还可能诱发其他的恶性肿瘤。Objective： To study the relationship between the infection of HCV in extrahepatic tissues and extrahepatic tumors of chronic hepatitis C patients. Methods ： Using the technique of immunohistochemistry to detect the HCV antigen in the operation specimens of the livers and extrahepatic tissues of chronic hepatitis C, and adopting RT-PCR to test plus strand and minus strand HCV RNA. Results： The tissue samples with malignant tumors were 37, the positive rate of HCV antigen was 67.6% （25/37） ; with non-malignant diseases were 33, the positive rate of HCV antigen was 24. 2% （8/33）, which had significant difference between the two groups （Х^2 = 13.14, P 〈 0.05 ） . For the 33 HCV antigen positive tissues, the rates of the plus strand and minus strand HCV RNA were 63. 6% （21/33）, 30. 3% （10/33） respectively. Conclusion： HCV can infect extrahepatic tissues, and parts of extrahepatic tissues are the replication sites for HCV, in addition, HCV could induce either primary hepatic carcinoma or other malignant tumors.

关 键 词：肝炎 丙型 慢性 肝外组织 免疫组织化学 逆转录聚合酶链反应 

分 类 号：R512.63[医药卫生—内科学]