毛蚶中抗癌活性肽高效液相指纹图谱分析研究(英文)  

Fingerprint Analysis of Anti-tumor Active Polypeptides from Arca subcrenata by HPLC

作　　者：任胜芳[1] 宋丽艳[1] 严春艳[1] 李婷菲[1] 赵昱[2] 于荣敏[1] 

机构地区：[1]暨南大学药学院,广东广州510632 [2]浙江大学药学院,浙江杭州310058

出　　处：《中药材》2008年第8期1134-1138,共5页Journal of Chinese Medicinal Materials

基　　金：广东省科技计划重大专项(2004A30505003);广州市科技计划重点项目(2005Z2-E4031)

摘　　要：应用RP-HPLC技术分析毛蚶中的抗癌活性肽。毛蚶活性肽的最优分离条件为:温度:30℃;波长:280nm;流速:1.0 ml/min;流动相A为80%乙腈(含0.1%TFA),流动相B为0.1%TFA水溶液,梯度洗脱。应用上述条件,成功地得到了10批样品的指纹图谱,并确定了14个共有峰。RP-HPLC was applied to analyze active polypeptides in Area subcrenata, and the optimal condition for separation was also set up ： temperature ： 30℃ ; wavelength ： 280 nm; flow rate ： 1.0 ml/min ; Solvent A consisted of 80% acetonitrile and 0. 1% trif- luoroacetic acid（TFA） and solvent B contained 0. 1% TFA. In this condition, ten samples＇ fingerprints were gained, in one of which the genuine fraction exhibited fourteen ＂common peaks＂ representing the characteristics of the constituents.

关 键 词：毛蚶 反相高效液相色谱 指纹图谱 鉴定 

分 类 号：R282.77[医药卫生—中药学]