黄芪对单侧输尿管梗阻大鼠肾组织结缔组织生长因子表达的影响  

Effect of Astragalus mongholicus on Expression of Connective Tissue Growth Factor in Kidney of SD Rats with Unilateral Ureteral Obstruction

作　　者：左川[1] 谢席胜[1] 邓尧[1] 樊均明[1] 

机构地区：[1]四川大学华西医院肾内科,四川成都610041

出　　处：《中药材》2008年第8期1185-1190,共6页Journal of Chinese Medicinal Materials

摘　　要：目的:动态观察黄芪(AM)对单侧输尿管梗阻(UUO)所致大鼠肾小管间质纤维化的拮抗作用及对结缔组织生长因子(CTGF)表达的影响,初步探讨其可能的分子作用机制。方法:SD大鼠随机分为假手术(Sham)、UUO组和UUO+AM治疗组(AM组)。在造模后第7、14 d处死动物,观察肾脏病理改变。采用免疫组化方法检测肾组织中CTGF和α-平滑肌肌动蛋白(α-SMA)表达。用实时荧光定量RT-PCR检测大鼠CTGF mRNA和α-SMA mRNA表达量。用Western blot检测大鼠CTGF蛋白表达量。结果:与假手术组相比,各时间点的UUO大鼠肾脏病理损害进行性加重,CTGF和α-SMA mRNA和蛋白表达随梗阻时间延长逐渐增高;黄芪治疗后可明显减轻UUO大鼠肾组织的肾小管损伤及肾间质纤维化,使UUO大鼠肾组织中高表达的CTGF和α-SMA降低(P<0.05)。结论:黄芪可能从核酸和蛋白水平抑制CTGF的表达,抑制肾小管上皮细胞转分化,减轻和改善UUO大鼠肾间质纤维化。Objective： To study the effect ofAstrangalus mongholicus（AM） on the expression of Connective Tissue Growth Factor （CTGF） in SD rats with Unilateral Ureteral Obstruction （UUO） and elucidate the mechanism underlying the renorotective effects of AM. Methods： 36 Sprague-Dawley rats were randomly divided into 3 groups： sham-operation group（ Sham）, UUO group（UUO） and UUO ＋ AM group （AM）. After administration of AM （ 10 g/kg · d） for 7 and 14 days, the dynamic histological changes of renal intersti- tial tissues were observed and renal damage including tubular impairment and interstitial fibrosis were quantified on HE and Masson stained tissue sections. The expressions of CTGF and α-smooth muscle actin（α-SMA） were measured by immunohistochemistry staining sections. The mRNA of CTGF and α-SMA were reversely transcribed and quantified to real-time PCR. The expression of CTGF protein was assessed by Western blot. Results ： Renal damage was exacerbated and the expressions of α-SMA and CTGF significantly increased in UUO group compared with those of Sham group （ P 〈 0. 05） at each time point. Tubular impairment and interstitial fibrosis were alle- viated, and up-regulations of expressions of CTGF and α-SMA were significantly depressed by AM treatment（P 〈 0. 05）. Conclusions ： AM can ameliorate renal interstitial fibrosis induced by UUO in rats. The mechanism of its antifibrotic effects may be related to the down-regulation of CTGF expression, following suppression of tubulo-epithelial mesenchymal transdifferentation in renal intersitial pro- gress.

关 键 词：黄芪 转分化 结缔组织生长因子 单侧输尿管梗阻 

分 类 号：R285.5[医药卫生—中药学]