原因不明习惯性流产患者细胞免疫状态和Th1/Th2细胞因子的变化  

Changes of Th1/Th2 cytokines and immunological reaction state in unexplained recurrent spontaneous abortion women

作　　者：曹颖平[1] 张旸[1] 王梅华[1] 张敬喜[1] 陈志星[1] 张勇[2] 

机构地区：[1]福建医科大学附属协和医院检验科,350001 [2]上海交通大学医学院上海市免疫学研究所

出　　处：《福建医药杂志》2008年第4期6-8,共3页Fujian Medical Journal

基　　金：福建省自然科学基金课题(项目号C0510016)

摘　　要：目的探讨原因不明习惯性流产(URSA)患者T细胞、NK细胞亚群和Th1/Th2细胞因子的变化。方法用流式细胞仪测定65例URSA患者和30例正常妊娠妇女外周血CD3+、CD4+、CD8+、CD16+CD56+细胞亚群比例,同时用ELISA法测定两组外周血Th1/Th2细胞因子的含量。结果与正常妊娠组相比,URSA患者CD4+、CD4+/CD8+、CD16+CD56+百分比较高,而CD8+较低(P<0.05);URSA组Th1细胞因子水平高于正常妊娠组,而Th2细胞因子则相反(P<0.05)。结论T淋巴细胞、NK细胞亚群比例的改变和Th1/Th2极化与URSA的发生有关。Objective To investigate the changes of T lymphocytes subsets and Th1/Th2 related cytokines in unexplained recurrent spontaneous abortion （URSA） women. Methods The flow cytometry （FCM） was used to detect CD3^＋ , CD4^＋, CD8^＋ T lymphocyte and CD16^＋ CD56^＋ Natural Killer （NK） cell subsets and the ratio of CD4^＋/CD8^＋ of peripheral blood （PBL） ; ELISA was used to detect the concentrations of Th1/Th2 cytokines. A total of 65 cases of URSA patients were studied and 30 cases of normal fertiled women as the control group. Results The percentages of CD4^＋ , CD16^＋ CD56^＋ cell sub- sets and the ratio of CD4^＋/CD8^＋ as well as Th1 related cytokines of the URSA patients were significantly higher than those of normal fertiled women （P〈0.05）. Whereas the percentages of CD8^＋ cell subsets and Th2 related cytokines were significantly lower than those of normal fertiled women （P〈0.05）. Conclusion The changes in the percentages of T lympyocyte and NK cell subsets and the polarization of Th1/Th2 subset have something to do with URSA.

关 键 词：原因不明习惯性流产 T细胞亚群 TH1/TH2细胞因子 

分 类 号：R714.21[医药卫生—妇产科学]